免疫原性细胞死亡（ICD）途径在肺腺癌（LUAD）治疗中起到重要的预后作用。环状GMP-AMP合成酶（cGAS）-干扰素基因刺激物（STING）途径是驱动ICD激活的上游机制，但中心基因的相互作用尚不清楚。本研究旨在调查与ICD和cGAS-STING途径相关的中心基因。还研究了中心基因和相关基因本体（GO）术语的预后表现。从基因表达文库（GEO）数据库中提取了ICD诱导和cGAS-STING途径激活样本的基因表达数据，并从The Cancer Genome Atlas（TCGA）中提取了接受药物治疗的LUAD患者的基因表达和临床数据。鉴定了差异表达基因（DEGs），并利用蛋白质相互作用（PPI）分析鉴定了中心基因。通过Kaplan-Meier（K-M）和COX分析确定了危险度风险（HR）评分。使用基因集富集分析（GSEA）鉴定相关的GO术语，并使用受试者工作特征（ROC）分析评估相关基因集的预后表现。在两个GEO数据集中鉴定出了22个DEGs，并通过PPI分析确定了六个中心基因。经过生存分析，选择了角蛋白6A（KRTA6A）和脂肪酸2-羟化酶（FA2H）作为中心基因。GSEA和ROC分析表明，KRTA6A和FA2H基因集在预后表现上没有差异。KRTA6A和FA2H是与LUAD中cGAS-STING相关的ICD诱导有关的中心基因。版权所有 © 2023，国际抗癌研究院（Dr.George J.Delinasios）保留所有权利。

The immunogenic cell death (ICD) pathway plays a crucial prognostic role in lung adenocarcinoma (LUAD) therapy. The cyclic GMP-AMP synthase (cGAS)-stimulator of interferon genes (STING) pathway is an upstream mechanism that drives ICD activation, but the interaction of hub genes remains unclear. The present study aimed to investigate the hub genes involved in ICD and the cGAS-STING pathway. The prognostic performance for hub genes and related Gene Ontology (GO) terms were also investigated.Gene expression data of ICD induction and cGAS-STING pathway activation samples were extracted from the Gene Expression Omnibus (GEO) database, and gene expression as well as clinical data of LUAD patients who received pharmaceutical therapy were extracted from The Cancer Genome Atlas (TCGA). Differentially expressed genes (DEGs) were identified, and protein-protein interaction (PPI) analysis was used to identify hub genes. Hazard risk (HR) scores were identified using Kaplan-Meier (K-M) and COX analyses. Gene set enrichment analysis (GSEA) was performed to identify the related GO terms, and receiver operating characteristic (ROC) analysis was used to evaluate the prognosis performance of the related gene sets.A total of 22 DEGs were identified in the two GEO datasets, and six hub genes were identified by PPI analysis. Keratin 6A (KRTA6A) and fatty acid 2-hydroxylase (FA2H) were selected as the hub genes after survival analysis. GSEA and ROC analysis indicated that there was no difference between the KRTA6A and FA2H gene sets on prognosis performance.KRTA6A and FA2H are hub genes associated with the induction of cGAS-STING-related ICD in LUAD.Copyright © 2023, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.