SRY-box containing gene 17 (SOX17) 在癌症的发生和发展中发挥关键作用，被认为是癌症诊断和治疗的潜在靶点。然而，SOX17 在癌症中的表达模式及其临床相关性仍然未知。在本研究中，我们通过对多种癌症类型中 SOX17 的表达模式进行研究，探讨了 SOX17 的表达与药物反应之间的关系。采用单细胞和批量 RNA-seq 分析，探究了 SOX17 的表达特征。分析结果经过 qPCR 和免疫组化实验证实。我们进行了生存分析、药物反应分析和共表达分析，以说明 SOX17 与临床结果的相关性。 结果显示，SOX17 的异常表达在多种癌症类型中高度异质性，表明 SOX17 是癌症类型相关的特征。此外，SOX17 的表达模式也与某些癌症类型的预后相关。强烈的 SOX17 表达与影响 PI3K/mTOR 信号通路的小分子药物的效力相关。与 SOX17 密切相关的基因 FGF18 参与了 PI3K-AKT 信号通路。单细胞 RNA-seq 分析表明，在卵巢癌的发展过程中，SOX17 主要表达在内皮细胞中，而在其他细胞中的表达数量较少，但在其他细胞类型中会扩散。 我们的研究通过批量和单细胞 RNA-seq 分析揭示了 SOX17 在全癌中的表达模式，并确定了其与某些肿瘤的诊断、分期和预后的相关性。这些发现具有临床意义，可以帮助我们找到可应用于药物干预的机制途径。 版权所有 © 2023，国际抗癌研究所（George J. Delinasios 博士），保留所有权利。

SRY-box containing gene 17 (SOX17) plays a pivotal role in cancer onset and progression and is considered a potential target for cancer diagnosis and treatment. However, the expression pattern of SOX17 in cancer and its clinical relevance remains unknown. Here, we explored the relationship between the expression of SOX17 and drug response by examining SOX17 expression patterns across multiple cancer types.Single-cell and bulk RNA-seq analyses were used to explore the expression profile of SOX17. Analysis results were verified with qPCR and immunohistochemistry. Survival, drug response, and co-expression analyses were performed to illustrate its correlation with clinical outcomes.The results revealed that abnormal expression of SOX17 is highly heterogenous across multiple cancer types, indicating that SOX17 manifests as a cancer type-dependent feature. Furthermore, the expression pattern of SOX17 is also associated with cancer prognosis in certain cancer types. Strong SOX17 expression correlates with the potency of small molecule drugs that affect PI3K/mTOR signaling. FGF18, a gene highly relevant to SOX17, is involved in the PI3K-AKT signaling pathway. Single-cell RNA-seq analysis demonstrated that SOX17 is mainly expressed in endothelial cells and barely expressed in other cells but spreads to other cell types during the development of ovarian cancer.Our study revealed the expression pattern of SOX17 in pan-cancer through bulk and single-cell RNA-seq analyses and determined that SOX17 is related to the diagnosis, staging, and prognosis of some tumors. These findings have clinical implications and may help identify mechanistic pathways amenable to pharmacological interventions.Copyright © 2023, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.