干扰素-γ（IFN-γ），通常称为II型干扰素，是一种关键的细胞因子，协调肿瘤免疫过程，在肿瘤免疫疗法研究中受到广泛关注。以往的研究已经讨论了IFN-γ在特定肿瘤或疾病中的作用和机制，但IFN-γ在广谱癌症中的相关作用仍不确定。我们下载了TCGA和GTEx的RNA表达数据和临床数据，并使用生物信息学方法分析了IFN-γ在肿瘤中的作用，包括IFN-γ在不同肿瘤中与表达、预后、功能状况、TMB、MSI、免疫细胞浸润和TIDE之间的相关性分析。我们还建立了PPI网络进行网状拓扑分析，将具有大于IFN-γ水平的度数的基因定义为中心基因。IFN-γ在TCGA的大多数肿瘤类型中表达差异显著，并且预测了不同的生存状况。此外，IFN-γ表达与多种因素密切相关，如T细胞浸润、免疫检查点、免疫激活基因、免疫抑制基因、趋化因子和趋化受体、肿瘤纯度、功能状态和预后价值。预后、CNV和治疗反应都与IFN-γ相关基因的表达显著相关。特别是，在宫颈内膜症中，IFN-γ相关基因STAT1的SNV百分比最高，SNP百分比也最高。IFN-γ相关基因表达水平升高在多种肿瘤类型中被发现，并且与20种不同类型的药物敏感性呈正相关。IFN-γ是肿瘤免疫疗法响应的良好指标，并且可能抑制肿瘤进展，为免疫疗法的未来发展提供了新的途径。版权所有 © 2023 魏、阮、张、覃、张、覃和李。

Interferon-gamma (IFN-γ), commonly referred to as type II interferon, is a crucial cytokine that coordinates the tumor immune process and has received considerable attention in tumor immunotherapy research. Previous studies have discussed the role and mechanisms associated with IFN-γ in specific tumors or diseases, but the relevant role of IFN-γ in pan-cancer remains uncertain.TCGA and GTEx RNA expression data and clinical data were downloaded. Additionally, we analyzed the role of IFN-γ on tumors by using a bioinformatic approach, which included the analysis of the correlation between IFN-γ in different tumors and expression, prognosis, functional status, TMB, MSI, immune cell infiltration, and TIDE. We also developed a PPI network for topological analysis of the network, identifying hub genes as those having a degree greater than IFN-γ levels.IFN-γ was differentially expressed and predicted different survival statuses in a majority of tumor types in TCGA. Additionally, IFN-γ expression was strongly linked to factors like infiltration of T cells, immune checkpoints, immune-activating genes, immunosuppressive genes, chemokines, and chemokine receptors, as well as tumor purity, functional statuses, and prognostic value. Also, prognosis, CNV, and treatment response were all substantially correlated with IFN-γ-related gene expression. Particularly, the IFN-γ-related gene STAT1 exhibited the greatest percentage of SNVs and the largest percentage of SNPs in UCEC. Elevated expression levels of IFN-γ-related genes were found in a wide variety of tumor types, and this was shown to be positively linked to drug sensitivity for 20 different types of drugs.IFN-γ is a good indicator of response to tumor immunotherapy and is likely to limit tumor progression, offering a novel approach for immunotherapy's future development.Copyright © 2023 Wei, Ruan, Zhang, Qin, Zhang, Qin and Li.