本研究旨在评估由螺旋藻（Spirulina platensis）提取物生物合成的纳米银颗粒（AgNPs）的抗氧化、抗癌和抗微生物活性。通过傅里叶变换红外（FTIR）分析、扫描电子显微镜（SEM）、透射电子显微镜（TEM）和X射线衍射（XRD）分析对生物合成的银纳米颗粒进行了表征。通过DPPH自由基清除实验确定了生物合成的AgNPs的抗氧化活性，而通过MTT实验确定了其抗癌活性。通过圆片扩散法分析了生物合成的AgNPs的抗微生物活性。螺旋藻充当还原和包覆剂。生物合成的AgNPs在500 g/ml浓度下表现出最强的抗氧化活性为80.89 ± 1.99，而在100µg/ml浓度下观察到最低的抗氧化活性为36 ± 1.97。MTT结果显示，生物合成的AgNPs的细胞毒性与浓度相关（对HepG2和A549细胞系均是如此）。此外，观察到生物合成的AgNPs对铜绿假单胞菌具有显著的抗菌活性，而对醋酸杆菌的抗菌活性最低。总之，我们从螺旋藻（Spirulina Platensis）中生物合成的AgNPs具有潜在的抗氧化、抗癌和抗菌活性。© 2023 Wiley-VCH GmbH.

The current study was designed to evaluate the antioxidant, anticancer and antimicrobial activities of silver nanoparticles (AgNPs) biosynthesized by Spirulina platensis extract. The biosynthesized silver nanoparticles were characterized using fourier transform infrared (FTIR) analysis, scanning electron microscopy (SEM), transmission electron microscopy (TEM) and X-ray diffraction (XRD) analysis. The antioxidant activity of the biosynthesized AgNPs were determined via DPPH radical scavenging assay while its anticancer activity was determined using the MTT assay. The antimicrobial activity of the biosynthesized AgNPs were analyzed by disc diffusion method. Spirulina platensis acts as a reducing and capping agent. The biosynthesized AgNPs exhibited strongest antioxidant activities of 80.89 ± 1.99 at 500 g/ml concentration while their lowest antioxidant activities of 36 ± 1.97 were observed at 100µg/ml concentration. MTT results showed that cytotoxicity of biosynthesized AgNPs was concentration dependent (for both HepG2 and A549 cell lines). Furthermore, significant antibacterial activity of the biosynthesized AgNPs was observed against pseudomonas aeroginosa while minimum antibacterial activity was observed against Acetobacter. In conclusion, our biosynthesized AgNPs from the algae Spirulina Platensis can be potent antioxidant, anticancer and antibacterial activities.© 2023 Wiley-VCH GmbH.