结直肠癌是癌症相关死亡的最常见原因之一，其主要转移部位是肝脏。对于结直肠癌转移至肝脏的手术方法因受影响的肝叶而异，预后也随之不同。然而，相关基础研究的缺乏使得我们需要一个良好的动物模型用于研究结直肠癌转移至肝脏不同肝叶的基础问题。 采用转染绿色荧光蛋白病毒的CT26结肠癌细胞系通过脾脏接种到BALB/C小鼠体内。根据门脉支的结扎情况和夹闭时间，在荧光显微镜下观察肝叶中的肿瘤形成情况。然后将不同肝叶的转移病灶差异与解剖结构进行比较。用血清样品检测术后肝功能的变化。 将CT26细胞注射后1分钟结扎并切除脾脏，脾切除术后夹闭血管1分钟后松开，可以建立一个对肝功能影响较小且符合要求的理想的肿瘤携带小鼠模型。选择性夹闭每个门脉支并经脾脏注射CT26细胞系，成功建立了一种具有明显特点的结直肠癌肝叶选择性肿瘤携带模型。 该模型为研究结直肠癌转移到肝脏不同肝叶的转移机制提供了机会，可能为临床治疗提供了基础。

Colorectal cancer is one of the most common causes of cancer-related death, and its main site of metastasis is the liver. The surgical method used for metastases of colorectal cancer in the liver varies according to the lobe affected, as does the prognosis. However, there is a lack of relevant basic research. Therefore, a good animal model is needed for basic studies of metastases from colorectal cancer to the different lobes of the liver.A CT26 colon cancer cell line transfected with a virus expressing green fluorescent protein was inoculated into BALB/C mice via the spleen. Tumor formation in the liver lobes was observed under a fluorescence microscope according to which portal vein branch was ligated and according to clamping time. The differential formation of metastatic lesions in the different lobes was then compared with physical anatomy. Serum samples were used to detect the changes in liver function postoperatively.Ligation and resection of the spleen 1 min after injection of the CT26 cells and release of the vessel clamp 1 min after splenectomy created an ideal tumor-bearing mouse model with little effect on liver function. Selective clamping of each portal vein branch and splenic injection of a CT26 cell line successfully established a selective liver lobe tumor-bearing model of colorectal cancer with distinct characteristics.This model provides an opportunity for investigation of the mechanisms of metastasis of colorectal cancer to different lobes of the liver and may provide a basis for clinical treatment.