背景：癌症发病率的增加意味着每两个人中就有一个人会被诊断出患有癌症。超过60％的癌症患者接受放射疗法，无论是作为独立的治疗还是与化疗和手术等其他治疗方法相结合。尽管放射疗法可以有效摧毁癌细胞，但它也会导致正常细胞和周围组织的后续损伤，原因是肿瘤微环境的改变和反应性氧化物（ROS）的增加。这可能会导致广泛的损害，损害组织功能，并且新的健康细胞无法在受损环境中存活，因此组织再生和功能恢复的可能性显着降低。在头颈部癌症治疗中，放射疗法可能导致唾液腺功能障碍。这会严重影响患者的生活质量，目前只有缓解治疗选项，没有治愈方法。组织工程方法可用于模拟组织的微环境，并通过生物活性化合物调节受损的微环境，以支持新的健康细胞在受损环境中的输送、存活和增殖。方法：在这项研究中，我们成功地将维生素A的衍生物醋酸视黄醇纳入了静电纺丝的聚己内酯纤维中。结果：扫描电镜图像和表征分析显示，所有制备的支架具有相似的特征，包括纤维形态和支架润湿性。体外实验表明，维生素支架具有抗氧化活性，通过清除DPPH和羟基自由基的活性。重要的是，这种抗氧化支架在正常条件和模拟氧化应激环境下培养的人颌下腺细胞的生长，并显着上调了抗氧化酶GPx1的表达。讨论：这些结果表明，将醋酸视黄醇纳入静电纺丝纤维中可能通过修复癌症放射治疗后的受损微环境。版权所有 © 2023 Westwood、Emmerson和Callanan。

Background: Increased cancer rates denote that one in two people will be diagnosed with cancer in their lifetime. Over 60% of cancer patients receive radiotherapy, either as a stand-alone treatment or in combination with other treatments such as chemotherapy and surgery. Whilst radiotherapy is effective in destroying cancer cells, it also causes subsequent damage to healthy cells and surrounding tissue due to alterations in the tumor microenvironment and an increase in reactive oxygen species (ROS). This can cause extensive damage that impairs tissue function, and the likelihood of tissue regeneration and restoration of function is significantly reduced as new healthy cells cannot survive in the damaged environment. In the treatment of head and neck cancers, radiotherapy can cause salivary gland dysfunction. This significantly impairs the patient's quality of life and there is currently no cure, only palliative treatment options. Tissue engineering approaches are used to mimic the microenvironment of the tissue and can mediate the damaged microenvironment via bioactive compounds, to support the delivery, survival, and proliferation of new, healthy cells into the damaged environment. Methods: In this study, retinyl acetate, a derivative of vitamin A, was successfully incorporated into electrospun polycaprolactone fibres. Results: SEM images and characterization analyses showed that all scaffolds produced had similar characteristics, including fiber morphology and scaffold wettability. The vitamin scaffolds were shown to exert an antioxidant effect through scavenging activity of both DPPH and hydroxyl radicals in vitro. Critically, the antioxidant scaffolds supported the growth of human submandibular gland cells and significantly upregulated the expression of GPx1, an antioxidant enzyme, when cultured under both normal conditions and under a simulated oxidative stress environment. Discussion: These results suggest that incorporation of retinyl acetate into electrospun fibres has may mediate the damaged microenvironment post cancer radiation therapy.Copyright © 2023 Westwood, Emmerson and Callanan.