衰老是心血管疾病发展的一个主要风险因素。柚皮苷（NRG）是一种源自柑橘类水果的黄酮类化合物。它具有广泛的药理学特性，包括抗氧化、抗炎和心脏保护作用。本研究旨在评估NRG对老年大鼠缺血/再灌注（I/R）损伤的心脏保护效应。在本研究中，使用每日剂量为150 mg/kg的D-半乳糖（D-GAL）连续8周诱导大鼠衰老。大鼠在与D-GAL共同处理的同时口服NRG（40或100 mg/kg/day），持续8周。采用Langendorff独立心脏方法评估NRG对老年大鼠I/R损伤的影响。NRG治疗减少了老年动物心肌肥厚和最大挛缩水平。在缺血前期，NRG使心率下降恢复正常。在灌流期，NRG治疗改善了D-GAL对左心室舒张末压（LVDP）、心率压力乘积（RPP）以及左心室压力的最小和最大速率（±dp/dt）的影响。NRG还增强了离体心脏对心功能参数（±dp/dt和RPP）的缺血后恢复。NRG在老年大鼠中逆转了血清乳酸脱氢酶（LDH）、肌酸激酶MB同功酶（CK-MB）和肿瘤坏死因子阿尔法（TNF-α）水平的增加。它还使心脏组织中D-GAL降低的超氧化物歧化酶（SOD）活性恢复正常。NRG治疗减轻了老年心脏在I/R条件下的损伤。NRG可能通过抗氧化和抗炎机制改善衰老导致的心功能障碍。© 2023年。作者，由Springer-Verlag GmbH Germany独家许可，属于Springer Nature的一部分。

Aging is known as a main risk factor in the development of cardiovascular diseases. Naringin (NRG) is a flavonoid compound derived from citrus fruits. It possesses a wide spectrum of pharmacological properties, including antioxidant anti-inflammatory, and cardioprotective. This investigation aimed to assess the cardioprotective effect of NRG against the ischemia/reperfusion (I/R) injury in aged rats. In this study, D-galactose (D-GAL) at the dose of 150 mg/kg/day for 8 weeks was used to induce aging in rats. Rats were orally gavaged with NRG (40 or 100 mg/kg/day), in co-treatment with D-GAL, for 8 weeks. The Langendorff isolated heart was used to evaluate the effect of NRG on I/R injury in aged rats. NRG treatment diminished myocardial hypertrophy and maximum contracture level in aged animals. During the pre-ischemic phase, reduced heart rate was normalized by NRG. The effects of D-GAL on the left ventricular end diastolic pressure (LVDP), the rate pressure product (RPP), and the minimum and maximum rate of left ventricular pressure (±dp/dt) improved by NRG treatment in the perfusion period. NRG also enhanced post-ischemic recovery of cardiac functional parameters (± dp/dt, and RPP) in isolated hearts. An increase in serum levels of the lactate dehydrogenase (LDH), the creatine kinase-MB (CK-MB), and the tumor necrosis factor-alpha (TNF-α) were reversed by NRG in aged rats. It also normalized the D-GAL-decreased the superoxide dismutase (SOD) activity in the heart tissue. NRG treatment alleviated cardiac injury in aged hearts under conditions of I/R. NRG may improve aging-induced cardiac dysfunction through anti-oxidative and anti-inflammatory mechanisms.© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.