本研究探讨了抗氧化剂在光动力疗法（PDT）中的潜在应用。PDT引起的活性氧（ROS）介导的细胞死亡与抗氧化剂清除ROS正好相反。使用光敏剂与抗氧化剂光敏剂的结合可以弥补抗氧化剂使用导致的ROS损失，最终导致细胞死亡。在本工作中，我们将光敏剂IR 792盐酸盐染料与α-生育酚（A）和对香豆酸（C）封装在聚合物纳米载体（AC IR NPs）中，用于PDT和光热疗法（PTT）。我们报道了使用聚乳酸-聚乙二醇酸（PLGA）通过纳米沉淀法合成AC IR NPs。通过SEM和TEM观察到纳米颗粒的尺寸为80.4±15.6 nm，呈球形形态。合成的AC IR NPs在成纤维细胞系（L929）中表现出良好的生物相容性。此外，对乳腺癌细胞系（4T1）的体外制备纳米系统的功效评估显示近80%的显著细胞死亡。这可以归因于ROS的产生导致氧化应激和抑制转移。本研究提供了证据表明，抗氧化药物与光敏剂的组合在治疗乳腺癌方面具有潜在的有效性。© 2023 IOP Publishing Ltd.

The potential use of antioxidants for photodynamic therapy (PDT) is investigated in this study. PDT causes Reactive oxygen species (ROS)-mediated cell death; on the contrary, antioxidants scavenge ROS. The use of a photosensitizer along with an antioxidant photosensitizer compensates for the loss of ROS due to the use of antioxidant, eventually leading to cell death. In this work, for PDT and photothermal therapy (PTT), we have combined the photosensitizer IR 792 Perchlorate dye with the antioxidants alpha-tocopherol (A) and p-coumaric acid (C) encapsulated in a polymeric nanocarrier (AC IR NPs). We have reported the synthesis of AC IR NPs using Poly Lactic-co-Glycolic Acid (PLGA) by nanoprecipitation method. The size of the polymeric nanoparticle were found to be 80.4 ± 15.6 nm, with a spherical morphology observed by SEM and TEM. The synthesized AC IR NPs demonstrated good biocompatibility in fibroblast cell lines (L929). Furthermore, the efficacy assessment of the as prepared nanosystem in vitro on breast cancer cell lines (4T1) revealed a significant cell death of nearly 80%. This could be attributed to the ROS generation leading to oxidative stress and inhibition of metastasis. This study provides an evidence that the combination of antioxidant drugs along with photosensitizers have the potential to be an effective therapy for treating breast cancer.© 2023 IOP Publishing Ltd.