β-半乳糖苷酶 (β-gal) 是一种典型的水解糖苷键的糖苷酶，被认为是细胞衰老和癌症发生的重要生物标志物。鉴于荧光成像技术具有高时空分辨率、高灵敏度、非侵入性和无电离辐射的优点，提供了在β-gal体内成像的绝佳选择。在本综述中，我们详细阐述了荧光成像探针在β-gal上的代表性生物技术进展，以揭示它们在临床前研究和临床应用中的未来潜力。接下来，我们提出了关于高保真度的β-gal成像探针的全面设计策略。考虑到系统实施方法，采用了一系列针对单个β-gal相关生物情境的高保真度成像引导治疗方法。最后，提出了当前的挑战和未来的趋势，以促进针对个体和特定应用场景的成像剂的下一步发展。版权所有 © 2023. Elsevier Inc. 出版。

Beta-galactosidase (β-gal), a typical glycosidase catalyzing the hydrolysis of glycosidic bonds, is regarded as a vital biomarker for cell senescence and cancer occurrence. Given the advantages of high spatiotemporal resolution, high sensitivity, non-invasiveness, and being free of ionizing radiations, fluorescent imaging technology provides an excellent choice for in vivo imaging of β-gal. In this review, we detail the representative biotech advances of fluorescence imaging probes for β-gal bearing diverse fidelity-oriented improvements to elucidate their future potential in preclinical research and clinical application. Next, we propose the comprehensive design strategies of imaging probes for β-gal with respect of high fidelity. Considering the systematic implementation approaches, a range of high-fidelity imaging-guided theragnostic are adopted for the individual β-gal-associated biological scenarios. Finally, current challenges and future trends are proposed to promote the next development of imaging agents for individual and specific application scenarios.Copyright © 2023. Published by Elsevier Inc.