炸油富含大量自由基，食用通过这种方法制备的食物会导致由氧化应激引起的神经组织损伤。由于中等强度的有氧运动训练能够减少氧化应激，本研究旨在调查有氧运动对炸油喂养大鼠海马组织中氧化应激、凋亡和神经生成标志物的影响。将18只雄性Wistar大鼠（200-280g）随机分为对照组（正常饮食），炸油对照组（喂养炸油）和炸油有氧运动组（炸油-运动）（每组6只）。连续四周，每周五天，给予2ml的炸油灌胃。有氧运动包括在跑步机上跑步，连续四周，每周五次，每次40分钟。通过PCR方法检测B细胞淋巴瘤2（BCL-2）基因、与Bcl-2相关的X蛋白（BAX）基因、Caspase-3（Casp-3）基因和Caspase-9（Casp-9）基因的表达。采用ELISA方法计算超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性、丙二醛（MDA）和脑源性神经营养因子（BDNF）水平。还通过免疫组织化学和TUNEL染色评估大鼠脑组织中Cannabinoid受体类型1（CB1）、Cannabinoid受体类型2（CB2）、胶质纤维酸性蛋白（GFAP）、神经核（NeuN）和DNA断裂的蛋白表达。结果发现，炸油喂养导致BAX、Casp-3、Casp-9基因表达和DNA断裂的显著增加（p≤0.05），同时降低了BDNF浓度和SOD活性（p≤0.05）。有氧运动显著降低了BAX、Casp-3、Casp-9、MDA、CB1、GFAP和DNA断裂（p≤0.05）。综上所述，有氧运动可以减少炸油喂养对海马组织的有害影响。版权所有 © 2023 Elsevier B.V. 发布。

Deep-frying oil (DFO) contains high amounts of free radicals, and consuming foods prepared with this method causes damage to nervous tissue due to oxidative stress (OS). Since moderate-intensity aerobic exercise training (AT) reduces OS, the current search investigated the effects of AT on OS, apoptosis, and neurogenesis markers in the hippocampal tissue of DFO-fed rats. Eighteen Wistar male rats (200-280 gr) were randomly allocated to a control group fed with normal food (Con-ND), a control group receiving DFO (Con-DFO), and a group receiving DFO-aerobic exercise (EX-DFO) (n= 6 in each). DFO was gavaged for four weeks, five days a week, with a dose of 2ml. AT included running on a treadmill for four weeks and five sessions per week (40minutes per session). The expression of genes B-cell lymphoma 2 (BCL-2), Protein X associated with Bcl-2 (BAX), Caspase-3 (Casp-3), and Caspase-9 (Casp-9) was measured by PCR method. The ELISA method was used to calculate levels of Superoxide dismutase (SOD) and Catalase (CAT) activity, malondialdehyde (MDA), and Brain-Derived Neurotrophic Factor (BDNF). Also, the expression of the proteins Cannabinoid receptor type 1(CB1), Cannabinoid receptor type2 (CB2), Glial fibrillary acidic protein (GFAP), Neuronal nuclei (NeuN), and DNA fragmentation was evaluated by Immunohistochemical and TUNEL staining. DFO feeding led to a significant increase in apoptotic markers, such as BAX, Casp-3, and Casp-9 gene expression, and DNA fragmentation (p≤0.05) while decreasing BDNF concentration SOD activity (p≤0.05). AT significantly reduced the BAX, Casp-3, Casp-9, MDA, CB1, GFAP, and DNA fragmentation (p≤0.05). In conclusion, AT can reduce the harmful effects of feeding with DFO on the hippocampal tissue.Copyright © 2023. Published by Elsevier B.V.