脂质体是一种独特的生物分子，能够装载亲水性和疏水性分子，并输送到生物系统中。本研究制备了以透明质酸（HA）和壳聚糖（CS）作为载体的脂质体（L），并封装了小檗碱（BER）和阿霉素（DOX），以增强药物在肺癌细胞中的增殖和治疗效果。通过傅立叶红外光谱（FTIR）、X射线衍射（XRD）、扫描电子显微镜（SEM）和透射电子显微镜（TEM）等技术，对功能团鉴定、结晶度和表面形态进行了分析。体外药物释放实验证实了BER和DOX在不同生理环境中的持续释放。HA-CS@BER&DOX-L在A549细胞内具有良好的穿透能力和较高的细胞毒性效应，其IC50值为99.70 μg/mL。纯脂质体的细胞毒性效应几乎可忽略不计，而HA-CS@BER&DOX-L可以有效诱导A549细胞凋亡。HA-CS@BER&DOX-L的细胞摄取分析显示其能够有效地靶向和进入A549细胞，并清晰观察到秀丽线虫图像。因此，本研究提出的体系有望成为增强A549癌细胞细胞毒性的潜在治疗方法，并有助于未来药物给药方法的发展。版权所有 © 2023 Elsevier B.V. 发布。

Liposomes are unique biomolecular, capable of loading both hydrophilic and hydrophobic molecules and delivered into the biological system. Liposomes (L) coated with hyaluronic acid (HA) and chitosan (CS) carrier system was fabricated. Berberine (BER) and doxorubicin (DOX) were encapsulated to enhance drug proliferation and therapeutic effect in lung cancer cells. The FTIR, XRD, SEM, and TEM techniques were carried out for functional group identification, crystallinity, and surface morphology analysis, respectively. In-vitro drug release confirms the sustained release of BER and DOX in various physiological environments. HA-CS@BER&DOX-L has good penetration ability and higher cytotoxicity effect in the A549 cells, and the IC50 value of HA-CS@BER&DOX-L is 99.70 μg/mL. The pure liposome showed a negligible cytotoxicity effect, and the HA-CS@BER&DOX-L could efficiently induce the apoptosis of A549 cells. The cellular uptake analysis of the HA-CS@BER&DOX-L effectively targeted and entered the A549 cells and clearly observed C. elegan images. Hence, the proposed system will be a potential treatment methodology to enhance the cytotoxicity of the A549 cancer cells and be useful to future drug administration methodology development.Copyright © 2023. Published by Elsevier B.V.