作为人体最大的器官，皮肤在阻挡环境损害方面起着关键作用。然而，许多因素，如遗传、阳光暴露和生活方式选择，都会导致皮肤损伤，从而产生皱纹、下垂和弹性丧失。含有天然成分的护肤品的使用越来越受欢迎，作为对抗衰老迹象的一种方式。鱼子酱油是一种因其富含脂肪酸、维生素和矿物质而受到关注的成分。本研究的目的旨在调查鱼子酱油的潜在抗衰老效果，并开发一种名为“鱼子油膏”的产品，该产品可能能减少皱纹和皮肤下垂。为评估鱼子酱油对3T3-L1细胞系的成脂作用，本研究采用离体模型。此外，还进行了一项离体研究，用人体皮肤组织研究了鱼子酱油对胶原蛋白和弹性蛋白的形成以及基质金属蛋白酶-1,2,9（MMP-1、MMP-2、MMP-9）的表达的影响。此外，还在5项临床研究中招募了102名受试者，评估了我们的产品“鱼子油膏”在面部和颈部皱纹、面部和眼部提拉以及各种皮肤参数（如皮肤水分、皮肤弹性、皮肤密度、皮肤紧致放松、皮肤透明度和皮肤更新）的抗衰老功效。鱼子酱油在体外实验中促进了脂肪细胞分化，并增加了细胞内的脂质积累。体外分析表明，鱼子酱油降低了MMP-1、MMP-2和MMP-9的表达水平，并增加了弹性蛋白和胶原蛋白I、III的形成。此外，在临床研究中，使用“鱼子油膏”一次即可改善皮肤参数，在使用四周后观察到更显著的效果。鱼子酱油具有显著的减缓皮肤衰老的作用，有潜力应用于抗衰老产品中。 版权所有 © 2023，国际抗癌研究所（George J. Delinasios博士），保留所有权利。

As the largest organ of the human body, the skin serves as a critical barrier against environmental damage. However, many factors, such as genetics, sun exposure, and lifestyle choices can lead to skin damage creating wrinkles, sagging, and loss of elasticity. The use of skincare products containing natural ingredients has become increasingly popular as a way to combat the signs of aging. Caviar oil is one such ingredient that has gained attention due to its rich composition of fatty acids, vitamins, and minerals. The objective of this study was to investigate the potential anti-aging effects of caviar oil and to develop a product, Cavi Balm, which could potentially reduce wrinkles and skin sagging.An in vitro model using the 3T3-L1 cell line was employed to assess the effect of caviar oil on adipocyte differentiation. An ex vivo study using human skin tissue was conducted to investigate the impact of caviar oil on collagen and elastin formation and the expression of matrix metalloproteinase-1,2,9 (MMP-1, MMP-2, MMP-9). Furthermore, 102 participants were enrolled in five clinical studies to evaluate the anti-aging efficacy of our product, "Cavi Balm", in facial and neck wrinkles, facial and eye area lifting, and various skin parameters, such as skin moisture, skin elasticity, skin density, skin tightening relief, skin clarity, and skin turnover.In vitro, caviar oil enhanced adipocyte differentiation, and increased lipid accumulation inside the cells. The ex vivo analysis revealed that caviar oil reduced the expression levels of MMP-1, MMP-2, and MMP-9, and increased the formation of elastin and collagen I, III. Moreover, in the clinical study, Cavi Balm improved skin parameters after one-time use, with more significant effects observed after four weeks of usage.Caviar oil has a substantial impact on mitigating skin aging and holds potential for application in anti-aging products.Copyright © 2023, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.