本研究旨在研究耳穴针刺治疗对实验微小猪运输应激的影响。实验1：将6只动物平均分成两组（对照组和治疗组）。治疗组在运输前（6小时；车辆和飞机）将短而超薄的圆形经皮针粘贴在双耳尖部穴位位置上。从头静脉开始，在运输前2天和运输后立即采集外周血样本，检测血压应激标志物、生化指标和氧化应激水平。实验2（随访研究：运输后腹泻发生率）：研究对照组和治疗组运输后腹泻发生率。实验1：运输应激导致血液皮质醇、血清淀粉样蛋白（SAA）、葡萄糖、非酯化脂肪酸和反应性氧代谢物（d-ROMs）及其相关物质增加，同时生物学抗氧化潜力（BAP）/d-ROMs比值减少，但不影响BAP水平。针灸抑制了SAA和d-ROMs值的增加以及BAP/d-ROMs比值的下降。实验2：对照组总腹泻发生率为25％，而治疗组无腹泻发生。针灸治疗抑制了下丘脑-垂体-肾上腺功能，从而减少了运输应激，但不影响中枢儿茶酚胺系统的抑制。通过针灸治疗运输应激可以改善动物福利。版权©2023，国际抗癌研究所（George J. Delinasios博士），保留所有权利。

This study aimed to investigate the effects of acupuncture treatment through the ear acupoints on transport stress in experimental microminipigs.Experiment 1: Six animals were equally divided into two groups (Control and Treatment). In the treatment group, before transportation (6 h; vehicle and plane), short, ultrathin circular transdermal needles were applied to locations corresponding to the acupoints on the apical area of both ears. Peripheral blood samples were collected from the cranial vena cava 2 days before and immediately after transportation. Blood stress markers, biochemistry indicators, and oxidative stress levels were examined. Experiment 2 (follow-up study: diarrhea incidence after transportation): Diarrhea incidence after transportation in the control and treatment groups was investigated.Experiment 1: Transport stress induced an increase in blood cortisol, serum amyloid A (SAA), glucose, non-esterified fatty acid, and derivatives of reactive oxygen metabolites (d-ROMs) and decreased the biological antioxidant potential (BAP)/d-ROMs ratio yet did not affect BAP. Acupuncture suppressed the increases in SAA and d-ROMs values and the decrease in BAP/d-ROMs ratio. Experiment 2: The total diarrhea incidence was 25% in the control group, whereas diarrhea was not observed in the treatment group.Acupuncture treatment suppresses hypothalamic-pituitary-adrenal function and, as a result, reduces transport stress without affecting the suppression of the central catecholaminergic system. Acupuncture treatment for transport stress can improve animal welfare.Copyright © 2023, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.