我们发现了一种新的生长因子，认为它是肝细胞癌（HCC）的生长刺激因子，它被命名为肝瘤衍生生长因子（HDGF）。最近，我们发现HDGF诱导了两种microRNA（miR-6072和miR-3137），这些microRNA也与HCC患者的预后相关。本研究旨在鉴定这些HDGF相关microRNA的靶基因。我们通过搜索公共数据库找到了与HDGF相关microRNA的候选靶基因。使用微阵列系统确定了在接受HDGF处理后表达发生变化的基因。最后，我们还在公共癌症基因组数据库中搜索到了由HDGF诱导的与HCC预后相关的基因。共鉴定了1,132个基因作为两种HDGF相关microRNA的共同靶基因。在这些基因中，微阵列系统显示，有6个基因的表达在接受HDGF处理后增加了（≥1.5倍）或减少了（≤0.67倍）。在癌症基因组数据库中，我们发现其中两个基因与HCC预后相关。高表达的烷基甘油磷酸合成酶（AGPS）与较差的生存率密切相关（1年、3年和5年生存率的p值分别为0.0025、0.0063和0.0081）。高表达的Shroom家族成员4（SHROOM4）基因与较好的生存率密切相关（1年、3年和5年生存率的p值分别为0.003、0.0006和0.0006）。本研究鉴定了与HCC预后相关的HDGF相关microRNA的潜在靶基因。版权所有 © 2023，国际抗癌研究学院（乔治·德里纳西奥斯博士），保留所有权利。

We identified a new growth factor, hepatoma-derived growth factor (HDGF), which is a presumed growth-stimulating factor of hepatocellular carcinoma (HCC). Recently, we identified two microRNAs (miR-6072 and miR-3137) induced by HDGF, which were also found to be associated with the prognosis of HCC patients. This study aimed to identify the target genes of these HDGF-related microRNAs.A public database was searched for candidate target genes of HDGF-related microRNAs. Using the microarray system, the genes whose expression changed in response to HDGF administration were determined. Finally, a public cancer genomics database was searched for genes that were induced by HDGF and associated with the prognosis of HCC.A total of 1,132 genes were identified as common target genes of the 2 HDGF-related microRNAs. Among these genes, a microarray system showed that the expression of 6 genes was increased (≥1.5-fold) or decreased (≤0.67-fold) after HDGF administration. Using a cancer genomics database, two of the six genes were found to be related to the prognosis of HCC. A high expression of alkylglycerone phosphate synthase (AGPS) was significantly associated with a poor survival (p=0.0025, 0.0063 and 0.0081 for the 1-, 3- and 5-year survival, respectively). A high expression of the shroom family member 4 (SHROOM4) gene was found to be significantly associated with a better survival (p=0.003, 0.0006 and 0.0006 for the 1-, 3- and 5-year survival, respectively).This study identified potential target genes of HDGF-related microRNAs that were associated with the prognosis of HCC.Copyright © 2023, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.