去泛素化酶3（DUB3）是泛素特异性蛋白酶家族的一个成员，参与调控细胞增殖、侵袭和凋亡。然而，DUB3表达的生物学作用和临床病理学意义尚未在非小细胞肺癌（NSCLC）中阐明。我们通过免疫组化技术评估了187例NSCLC患者（包括93例腺癌和72例鳞癌）的DUB3表达，并评估了其在临床病理学中的意义。在腺癌中，我们观察到DUB3表达对肿瘤大小（p=0.030）、血管浸润（p=0.038）、T期（p=0.014）和肿瘤复发（p=0.002）有影响。卡帕兰-迈尔曲线和log-rank检验显示，与低表达组相比，高DUB3表达与腺癌的临床预后显著相关（p=0.013）。无病生存的多因素分析还表明，DUB3表达是肺腺癌的独立预后因子（p=0.017）。另外，我们发现了DUB3与大肿瘤抑制激酶1（Hippo通路的核心蛋白）表达的相关性。DUB3在肺腺癌中通过调节Hippo通路发挥肿瘤抑制作用，并可被视为有强大预测因子和治疗靶点。版权所有 ©2023年，国际抗癌研究所（George J. Delinasios博士），保留所有权利。

Deubiquitinating enzyme 3 (DUB3) is a member of the ubiquitin-specific proteases family involved in regulating cell proliferation, invasion, and apoptosis. However, the biological role and clinicopathological significance of DUB3 expression have not been elucidated in non-small cell lung cancer (NSCLC).We evaluated the expression of DUB3 by immunohistochemistry using tissue microarrays and assessed the clinicopathologic significance of DUB3 expression levels in 187 patients with NSCLC, including its two major subtypes (93 cases of adenocarcinoma and 72 cases of squamous cell carcinoma).In adenocarcinoma, we observed that DUB3 expression had an effect on tumor size (p=0.030), vessel invasion (p=0.038), T stage (p=0.014), and tumor recurrence (p=0.002). Kaplan-Meier curves with log-rank test showed that high DUB3 expression was correlated with significantly more favorable clinical outcomes compared to those of the low expression group in adenocarcinoma (p=0.013). Multivariate analysis of disease-free survival also demonstrated that DUB3 expression is an independent prognostic factor in lung adenocarcinoma (p=0.017). Additionally, we identified the correlation between DUB3 and the expression of large tumor suppressor kinase 1 expression, a core protein of the Hippo pathway.DUB3 could function as a tumor suppressor by regulating the Hippo pathway in lung adenocarcinoma and can be considered a powerful predictive factor and therapeutic target.Copyright © 2023, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.