研究动态
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对突尼斯药用槐柳(学名:Tamarix africana Poir.)进行了植物化学研究,并评估了其抗氧化、抗炎和细胞毒性活性。

Phytochemical investigation, antioxidant, anti-inflammatory and cytotoxic activities of Tunisian medicinal Tamarix africana Poir.

发表日期:2023 Aug 31
作者: Manel Karker, Samia Oueslati, Hanen Falleh, Kamel Msaada, Jean Legault, Chedly Abdelly, André Pichette, Riadh Ksouri
来源: ANTIOXIDANTS & REDOX SIGNALING

摘要:

本研究旨在评估突尼斯秧柳(Tamarix africana Poir)的生物活性。在本研究中,对秧柳的新型生物活性进行了与其酚类相关的调查。研究了四种提取物(己烷、二氯甲烷、甲醇和水)的次生代谢物含量、抗氧化、抗炎和细胞毒性活性。通过体外和体内实验评估了抗氧化活性。此外,还对人肺癌(A-549)和结肠腺癌(DLD-1)细胞的抗癌活性进行了研究。通过抑制LPS诱导的RAW 264.7巨噬细胞系中的NO产生评估了抗炎能力。甲醇和水提取物显示出最高的抗氧化活性(IC50=3.3和4.3µg/mL),与其酚类含量相关。己烷提取物在80µg/mL时对NO活性表现出重要的抗炎效果,完全抑制了活性。此外,发现T. africana提取物对A-549肺癌细胞表现出活性,IC50值介于20到34µg/mL之间。这些结果表明,T. africana被认为是一种具有潜在效应对人类健康和疾病有益的易得天然分子的潜在来源。
The current study aimed to evaluate Tunisian Tamarix africana Poir biological activities. In this study, novel biological activities of the shoot extracts related to their phenolics investigated. Secondary metabolite contents, antioxidant, anti-inflammatory and cytotoxic activities of four extracts (hexane, dichloromethane, methanol and water) were investigated. Antioxidant activities were assessed via in vitro and ex vivo assays. Besides, anticancer activity was investigated against human lung carcinoma (A-549) and colon adenocarcinoma (DLD-1) cells. The anti-inflammatory ability was evaluated via inhibition of LPS-induced NO production in RAW 264.7 macrophage cell lines. Methanol and water extracts displayed the highest antioxidant (IC50 = 3.3 and 4.3 µg/mL respectively), which are correlated activities correlated with phenolic contents. Hexane extract exhibited an important anti-inflammatory effect inhibiting NO ability by 100% at 80 µg/mL. Besides, T. africana extracts were found to be active against A-549 lung carcinoma cells with IC50 values ranging from 20 to 34 µg/mL. These results suggested that T. africana is considered as a potential source of readily accessible natural molecules with a promising effect on human health and diseases.