基于抗体的成像质谱（IMC）是一种高度多重化的成像方法，可以捕获亚细胞分辨率下异质性空间蛋白质表达模式。我们在这里报道了通过DNA基于交换反应进行信号放大的IMC扩展到低丰度标记物的方法，即免疫-SABER。我们应用了SABER-IMC来同时对18个标记物进行免疫-SABER放大和20个标记物进行非放大的人类黑色素瘤肿瘤免疫微环境成像。SABER-IMC能够鉴定免疫细胞表型标记物，例如T细胞共受体及其配体，这些标记物在IMC中无法被检测到。© 2023. 作者。

Imaging mass cytometry (IMC) is a highly multiplexed, antibody-based imaging method that captures heterogeneous spatial protein expression patterns at subcellular resolution. Here we report the extension of IMC to low-abundance markers through incorporation of the DNA-based signal amplification by exchange reaction, immuno-SABER. We applied SABER-IMC to image the tumor immune microenvironment in human melanoma by simultaneous imaging of 18 markers with immuno-SABER and 20 markers without amplification. SABER-IMC enabled the identification of immune cell phenotypic markers, such as T cell co-receptors and their ligands, that are not detectable with IMC.© 2023. The Author(s).