采用行进表面声波（SAWs）在静止液滴内对癌细胞进行了溶解，无需任何化学试剂。然后进行拉曼光谱分析以探索详细的细胞衍生数据。利用互换型传感器产生的瑞利波使其在LiNbO3衬底表面传播。通过使用聚苯乙烯微粒（PSMPs）有效建立了机械细胞溶解，并添加了金纳米颗粒（AuNPs）以增强SAWs对溶解细胞的拉曼信号。通过实验中PSMPs的大小和浓度来评估溶解效率，并根据频率的变化进行了研究。在高频领域，溶解主要是通过PSMPs的机械碰撞进行的，并且通过增加SAWs的应用时间和能量密度来提高溶解效率。AuNPs形成的纳米间隙显著增强了溶解细胞的拉曼信号，而AuNPs的分布均匀，不受SAWs的频率无关行为的影响。最后，获取了MDA-MB-231（乳腺恶性癌细胞）的详细拉曼光谱，并观察到多种有机物衍生的峰值。与未经溶解处理的细胞相比，经过溶解处理的细胞的95%置信区分布更广。预计所提出的SAW平台能够便于检测少量物质并应用于生物医学领域。

The lysis of cancer cells inside a sessile droplet was performed using traveling surface acoustic waves (SAWs) without any chemical reagents. Raman spectrum profiling was then carried out to explore detailed cell-derived data. The Rayleigh waves formed by an interdigital transducer were made to propagate along the surface of an LiNbO3 substrate. Polystyrene microparticles (PSMPs) were used to establish mechanical cell lysis effectively, and gold nanoparticles (AuNPs) were added to enhance the Raman signals from the lysed cells by SAWs. The lysis efficiency was evaluated according to the size and concentration of the PSMPs in experiments where the frequency was varied. Lysis occurred mainly by mechanical collision using PSMPs in a high-frequency domain, and the lysis efficiency was improved by increasing the application time and the energy density of the SAWs. Raman signals from the lysed cells were greatly enhanced by nanogaps formed by the AuNPs, which were evenly distributed irrespective of the SAWs through the frequency-independent behavior of the AuNPs. Finally, detailed Raman spectra of MDA-MB-231, malignant breast cancer cells, were acquired, and various organic matter-derived peaks were observed. The 95% confidence region for cells subjected to lysis was more widely distributed than that of cells not subjected to lysis. The proposed SAW platform is expected to facilitate the detection of small quantities and to be applied in biomedical applications.