乳腺癌是全球重要的健康问题，寻找有效的治疗方法至关重要。癌症治疗的副作用，如手术、放疗和化疗，会降低患者的生活质量。最近，植物中的天然化合物因其安全性、低毒性和可能的疗效而引起了人们的关注，有潜力作为抗癌药物。石杉蕨菜（LC）是一种在热带地区和欧洲广泛分布的草本植物，以其多种药用特性而闻名。在本研究中，我们调查了LC水提取物（LC-WE）和LC乙醇提取物（LC-EE）对MCF-7人乳腺癌细胞的细胞毒性和凋亡效应。我们的结果显示，LC处理导致了对MCF-7细胞的剂量和时间依赖性的细胞毒性效应，表明其作为抗人乳腺癌药物的潜力。此外，我们观察到LC处理激活了与凋亡相关的蛋白质，包括BAX、Caspase-3和Caspase-9。这些结果表明，LC可能通过凋亡诱导机制来产生其对MCF-7乳腺癌细胞的细胞毒性效应。先前的研究已经显示了LC对不同类型的癌症具有抗癌潜力。然而，LC对人乳腺癌细胞的抗癌作用尚未得到研究。因此，我们的研究为LC作为抗乳腺癌药物的潜力提供了新的见解。总体而言，我们的结果强调LC作为一种有前景的天然化合物在乳腺癌治疗中的潜力。© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.

Breast cancer is a significant health problem worldwide, and the search for effective treatments is critical. Side effects of cancer treatments such as surgery, radiotherapy, and chemotherapy reduce the patient's standard of living. Recently, natural compounds from plants have gained attention as potential anticancer agents due to their safety, low toxicity, and potential efficacy. Lycopodium Clavatum (LC) is an herb abundant in tropical regions and Europe and is known for its various medicinal properties. In this study, we investigated the cytotoxic and apoptotic effects of LC Water Extract (LC-WE) and LC Ethanol Extract (LC-EE) plant extracts on MCF-7 human breast cancer cells. Our results showed that LC treatment led to a dose and time-dependent cytotoxic effect on MCF-7 cells, indicating its potential as an anticancer agent against human breast cancer. Additionally, we observed that LC treatment activated apoptosis-related proteins, including BAX, Caspase-3, and Caspase-9. These results suggest that LC may induce apoptosis as a mechanism underlying its cytotoxic effect on MCF-7 human breast cancer cells. Previous studies have shown the anti-cancer potential of LC against different types of cancer. However, the anti-cancer effect of LC on human breast cancer cells has not been investigated to date. Therefore, our study provides novel insights into the potential of LC as an anti-cancer agent against breast cancer. Overall, our results highlight the potential of LC as a promising natural compound for breast cancer treatment.© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.