肿瘤浸润淋巴细胞（TILs）和第三淋巴结结构（TLSs）在肿瘤组织中的存在与多种恶性肿瘤的预后有关。与此同时，中性粒细胞与淋巴细胞比值（NLR）作为一种全身性炎症标志物也与其预后相关。然而，关于肉瘤肺转移与这些生物标志物之间的关系还缺乏研究。我们在冈山大学医院回顾性招募了2006年1月至2019年12月期间进行肺转移切除术的102例子宫平滑肌肉瘤肺转移患者。通过免疫组化染色评估术后切除的肺转移标本中的TILs和TLSs，使用抗CD3 / CD8 / CD103 / Foxp3 / CD20抗体。 NLR是根据最近一次肺转移切除术前的血液检查计算的。我们阐明了这些因素与预后之间的关系。由于我们认为肿瘤组织的状况和全身性炎症同样有价值，我们还评估了TILs或TLSs和NLR的组合对预后的影响。关于TILs，CD3阳性细胞和CD8阳性细胞与预后相关。在CD8高组、CD3高组、TLSs高组和NLR低组的患者中，预后显著较好。CD8高/NLR低组和TLSs高/NLR低组的预后显著优于CD8低/NLR高组和TLSs低/NLR高组。CD3阳性TILs、CD8阳性TILs、TLSs和NLR分别与预后相关。CD8阳性TILs或TLSs与NLR的组合可能是预测子宫平滑肌肉瘤肺转移患者预后的指标。© 2023。作者。

The presence of tumor-infiltrating lymphocytes (TILs) and tertiary lymphoid structures (TLSs) in tumor tissue has been related to the prognosis in various malignancies. Meanwhile, neutrophil-to-lymphocyte ratio (NLR) as a systemic inflammation marker also has been associated with the prognosis in them. However, few reports have investigated the relationship between pulmonary metastases from sarcoma and these biomarkers.We retrospectively recruited 102 patients undergoing metastasectomy for pulmonary metastases from uterine leiomyosarcoma at Okayama University Hospital from January 2006 to December 2019. TILs and TLSs were evaluated by immunohistochemical staining of surgically resected specimens of pulmonary metastases using anti-CD3/CD8/CD103/Foxp3/CD20 antibodies. NLR was calculated from the blood examination immediately before the most recent pulmonary metastasectomy. We elucidated the relationship between the prognosis and these factors. Because we considered that the status of tumor tissue and systemic inflammation were equally valuable, we also assessed the impact of the combination of TILs or TLSs and NLR on the prognosis.As for TILs, CD3-positive cells and CD8-positive cells were correlated with the prognosis. The prognosis was significantly better in patients with CD3-high group, CD8-high group, TLSs-high group, and NLR-low group, respectively. The prognosis of CD8-high/NLR-low group and TLSs-high/NLR-low group was significantly better than that of CD8-low/NLR-high group and TLSs-low/NLR-high group, respectively.CD3-positive TILs, CD8-positive TILs, TLSs, and NLR are correlated with the prognosis, respectively. The combination of CD8-positive TILs or TLSs and NLR may be the indicators to predict the prognosis of patients with pulmonary metastases from uterine leiomyosarcoma.© 2023. The Author(s).