蓝莓和苹果由于富含丰富的植物化学物质，表现出有利的生物活性和保健功效。自然植物化学物质以复杂形式存在，但对于不同植物化学物质之间是否存在加成、协同或拮抗效应的报道较少。本研究的目的是阐明蓝莓提取物（BE）和苹果皮提取物（APE）在抑制肝癌HepG2细胞增殖方面的协同效应。同时，通过HPLC检测BE和APE的植物化学成分，并通过细胞抗氧化活性（CAA）测定、氧自由基吸收能力（ORAC）测定和过氧自由基清除能力（PSC）测定来确定总抗氧化活性。结果显示，BE和APE富含植物化学物质并具有强大的抗氧化活性，在协同作用下抑制细胞增殖。在双侧组合中，剂量减少指数（DRI）值增加了2倍，并且95%抑制时的组合指数（CI）值小于1。此外，BE+APE补充剂可促进p53和c-myc基因的表达水平。总之，BE和APE具有强大的抗氧化活性，并对HepG2细胞的增殖表现出协同抑制作用。这可以为健康护理中不同植物化学物质的协同作用提供理论依据。本文受版权保护。保留所有权利。

Blueberries and apples exhibit favorable bioactivity and health benefits due to their rich phytochemicals. Natural phytochemicals exist in complex forms, but there are few reports on whether have additive, synergistic or antagonistic effects between different phytochemicals. The aim of the present investigation was to elucidate the synergistic effects of blueberry extract (BE) and apple peel extract (APE) together in inhibiting the proliferation of HepG2 liver cancer cells. Meanwhile, phytochemical characterization of BE and APE were detected by HPLC, and total antioxidant activity was determined via cellular antioxidant activity (CAA) assay, oxygen radical absorption capacity (ORAC) assay and peroxy radical scavenging capacity (PSC) assay.The results showed that BE and APE were rich in phytochemicals and had potent antioxidant activities, which synergistically inhibited cell proliferation. In the bilateral combination, the dose reduction index (DRI) value increased 2-fold, and the combination index (CI) value at 95% inhibition was less than 1. Additionally, BE+APE supplementation could promote the expression levels of p53 and c-myc genes. In conclusion, the BE and APE had strong antioxidant activity and exhibited synergistic inhibition against HepG2 cells proliferation.This can provide a theoretical basis for the synergistic effect of different phytochemicals in health care. This article is protected by copyright. All rights reserved.This article is protected by copyright. All rights reserved.