用流式细胞术-荧光原位杂交（FACS-FISH）能够实现更高的浆细胞FISH检测率。
Superior detection rate of plasma cell FISH using FACS-FISH.
发表日期：2023 Sep 02
作者：
Marie-France Gagnon, Sally M Midthun, James A Fangel, Cynthia M Schuh, Ivy M Luoma, Kathryn E Pearce, Reid G Meyer, Sikander Ailawadhi, Mariano J Arribas, Esteban Braggio, Rafael Fonseca, S Vincent Rajkumar, Cinthya Zepeda-Mendoza, Xinjie Xu, Patricia T Greipp, Michael M Timm, Gregory E Otteson, Min Shi, Dragan Jevremovic, Horatiu Olteanu, Jess F Peterson, Rhett P Ketterling, Shaji Kumar, Linda B Baughn
来源：
AMERICAN JOURNAL OF CLINICAL PATHOLOGY
摘要：
荧光原位杂交（FISH）用于浆细胞肿瘤（PCNs）需要浆细胞（PC）识别或纯化策略以优化结果。我们在临床实验室环境中比较了细胞质免疫球蛋白FISH（cIg-FISH）和流式细胞分选FISH（FACS-FISH）的功效。分析了2019年至2022年间，经过细胞遗传学评估的怀疑有PCN的个体的14,855个样本的FISH分析结果，其中cIg-FISH测试组（n=6917）和FACS-FISH测试组（n=7938）。与cIg-FISH相比，流式细胞分选-FISH提高了异常检测率，分别在54%和50%的病例中记录到异常结果（P<0.001）。它改善了IGH::CCND1（P<0.001）、IGH::MAF（P<0.001）、IGH::MAFB（P<0.001）、其他IGH重排（P<0.001）和1q的增益/扩增（P<0.001）的检测率，而IGH::FGFR3融合（P=0.3）、17p缺失（P=0.3）和其他异常，包括高倍体性（P=0.5），的检测率相似。cIg-FISH和FACS-FISH的FISH分析PC产量不足分别降低了（22%和3%，P<0.001）。流式细胞术在91%的病例中确定了倍性状态。此外，FACS-FISH减少了分析时间、工作量和运营成本。荧光细胞分选-FISH是一种高效的PC纯化策略，能显著改善诊断产量，并降低工作流程要求，与cIg-FISH相比。© 2023作者。由牛津大学出版社代表美国临床病理学会发表。版权所有。需要权限，请发送电子邮件至：journals.permissions@oup.com。
Fluorescence in situ hybridization (FISH) for plasma cell neoplasms (PCNs) requires plasma cell (PC) identification or purification strategies to optimize results. We compared the efficacy of cytoplasmic immunoglobulin FISH (cIg-FISH) and fluorescence-activated cell sorting FISH (FACS-FISH) in a clinical laboratory setting.The FISH analysis results of 14,855 samples from individuals with a suspected PCN subjected to cytogenetic evaluation between 2019 and 2022 with cIg-FISH (n = 6917) or FACS-FISH (n = 7938) testing were analyzed.Fluorescence-activated cell sorting-FISH increased the detection rate of abnormalities in comparison with cIg-FISH, with abnormal results documented in 54% vs 50% of cases, respectively (P < .001). It improved the detection of IGH::CCND1 (P < .001), IGH::MAF (P < .001), IGH::MAFB (P < .001), other IGH rearrangements (P < .001), and gains/amplifications of 1q (P < .001), whereas the detection rates of IGH::FGFR3 fusions (P = .3), loss of 17p (P = .3), and other abnormalities, including hyperdiploidy (P = .5), were similar. Insufficient PC yield for FISH analysis was decreased between cIg-FISH and FACS-FISH (22% and 3% respectively, P < .001). Flow cytometry allowed establishment of ploidy status in 91% of cases. In addition, FACS-FISH decreased analysis times, workload efforts, and operating costs.Fluorescence-activated cell sorting-FISH is an efficient PC purification strategy that affords significant improvement in diagnostic yield and decreases workflow requirements in comparison with cIg-FISH.© The Author(s) 2023. Published by Oxford University Press on behalf of American Society for Clinical Pathology. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.