本研究旨在调查甘草酸（GL）对顺铂在A2780卵巢癌细胞中的抗癌活性的影响。将培养的A2780细胞分别用不同浓度的GL和顺铂以及二者的组合进行处理。采用MTT试验、流式细胞术、伤口愈合实验和克隆形成试验来确定细胞存活率、凋亡、迁移和集落形成情况。还评估了GL对过氧化物酶（SOD）活性的影响。采用基因定量PCR研究了GL和顺铂的单独和联合治疗对迁移基因（MMP2和MMP9）以及某些凋亡通路基因（caspase-3、-8、-9和BCL2）表达水平的影响。观察到GL和顺铂之间存在协同作用（CI<1）。与单独治疗相比，联合治疗在降低细胞存活率、抑制迁移和集落形成、诱导凋亡以及改变基因表达方面显著更有效。GL显著增加了SOD活性。处理细胞中caspase-3、-8和-9的相对表达显著增加，而MMP2和MMP9的表达水平显著降低。我们的结果表明，GL增强顺铂在A2780细胞系中的抗癌活性。因此，GL和顺铂的联合治疗可提议作为一种有前景的卵巢癌治疗策略。版权所有 © 2023 Elsevier Ltd.发表。

This study aimed to investigate the effects of glycyrrhizic acid (GL) on the anticancer activity of cisplatin in A2780 ovarian cancer cells. Cultured A2780 cells were treated with different concentrations of GL and cisplatin individually and in combination. The MTT assay, flow cytometry, wound-healing, and clonogenic assay, were used to determine cell viability, apoptosis, migration, and colony formation, respectively. The effects on superoxide dismutase (SOD) activity were also evaluated. QPCR was used to study the effects of individual and combined treatments with GL and cisplatin on the expression levels of migration genes (MMP2 and MMP9), and some apoptosis pathway genes (caspase-3, -8, -9, and BCL2). A synergistic effect was observed between GL and cisplatin (CI < 1). Combination therapy was significantly more effective in reducing cell viability, suppressing migration and colony formation, inducing apoptosis, and altering gene expression compared to single therapies. GL significantly increased SOD activity. The relative expression of caspase -3, -8, and - 9 increased significantly, and the expression levels of MMP2 and MMP9 decreased significantly in the treated cells. Our results indicate that GL enhances the anticancer activity of cisplatin in the A2780 cell line. Therefore, the combination of GL and cisplatin can be proposed as a promising therapeutic strategy for ovarian cancer.Copyright © 2023. Published by Elsevier Ltd.