Opa相互作用蛋白5（OIP5）是一种癌特异性基因，它在各种人类癌症中起到促癌作用。然而，OIP5在卵巢癌的致癌过程和进展中的作用尚不明确。我们首先使用Sangerbox在线分析工具分析了OIP5在卵巢癌和各种人类肿瘤中的表达情况。我们从基因表达全库（Gene Expression Omnibus，简称GEO）数据库下载了GSE12470、GSE14407和GSE54388，使用GEO2R筛选卵巢癌组织中的差异表达基因。使用基因本体（Gene Ontology，简称GO）富集分析来研究相关的生物过程。生成接受者操作特征曲线（Receiver Operating Characteristic，简称ROC）来评估OIP5对卵巢癌的预测能力。接下来，我们利用RT-PCR、免疫组织化学和蛋白质印迹法评估OIP5在卵巢癌中的表达情况。使用CCK8、EdU增殖实验和集落形成实验来测量细胞增殖，使用PI染色和流式细胞术来检测细胞周期进展，使用Caspase3/7活性实验来检测细胞凋亡。使用Transwell实验分析OIP5对卵巢癌细胞的迁移和侵袭能力的影响。我们发现通过生物信息学分析OIP5在卵巢癌中高度表达，并且重要的是，OIP5可能是卵巢癌预后和诊断的重要生物标志物。 RT-PCR实验、免疫组织化学和蛋白质印迹法也用于确认OIP5在卵巢癌中的高表达。随后，我们证明了在OIP5基因沉默后，卵巢癌细胞系A2780的增殖和迁移明显受到抑制，细胞凋亡增加并且细胞周期在G1期停滞。本研究表明OIP5在卵巢癌中高表达，并且OIP5的下调抑制了卵巢癌细胞的增殖、迁移和侵袭，诱导了细胞周期阻滞和促进了细胞凋亡。因此，OIP5可能是卵巢癌早期诊断的重要生物标志物，也是潜在的治疗靶点。 (c) 2023、BioMed Central Ltd.、Springer Nature的一部分。

Opa interacting protein 5 (OIP5), which is a cancer/testis-specific gene, plays a cancer-promoting role in various types of human cancer. However, the role of OIP5 in the carcinogenesis and progression of ovarian cancer remains unknown.We first analyzed the expression of OIP5 in ovarian cancer and various human tumors with the Sangerbox online analysis tool. GSE12470, GSE14407 and GSE54388 were downloaded from the Gene Expression Omnibus (GEO) database, and GEO2R was used to screen differentially expressed genes in ovarian cancer tissues. Gene Ontology (GO) enrichment analysis was used to explore the related biological processes. Receiver operating characteristic (ROC) curve was generated to evaluate the predictive ability of OIP5 for ovarian cancer. Next, RT-PCR, immunohistochemistry and Western blotting were utilized to evaluate the expression of OIP5 in ovarian cancer. CCK8, EdU proliferation assays and colony formation assays were used to measure cell proliferation, cell cycle progression was examined by PI staining and flow cytometry, and cell apoptosis was examined by Caspase3/7 activity assays. The effect of OIP5 on the migration and invasion of ovarian cancer cells was analyzed with Transwell assays.We found that OIP5 is highly expressed in ovarian cancer through bioinformatics analysis, and importantly, OIP5 may be an important biomarker for the prognosis and diagnosis of ovarian cancer. RT-PCR assays, immunohistochemistry and Western blotting were also used to confirm the high expression of OIP5 in ovarian cancer. Subsequently, we demonstrated that the proliferation and migration of the ovarian cancer cell line A2780 were significantly inhibited after OIP5 gene silencing, apoptosis was increased and cell cycle progression was arrested at the G1 phase.This study indicated that OIP5 was highly expressed in ovarian cancer and that downregulation of OIP5 inhibited the proliferation, migration and invasion of ovarian cancer cells, induced cell cycle arrest and promoted cell apoptosis. Therefore, OIP5 may be an important biomarker for the early diagnosis and potential target for treatment of ovarian cancer.© 2023. BioMed Central Ltd., part of Springer Nature.