来自棕藻的甘露聚糖和叶绿素对体外抗癌潜力的研究
In vitro anticancer potential of laminarin and fucoidan from Brown seaweeds.
发表日期：2023 Sep 02
作者：
Elumalai Sanniyasi, Rajesh Kanna Gopal, Rajesh Damodharan, Arthi Arumugam, Madhumitha Sampath Kumar, Nandhini Senthilkumar, Monisha Anbalagan
来源：
Cell Death & Disease
摘要：
海洋海藻是细胞壁中富含的多糖的丰富来源，在中国、日本、韩国和南亚国家进行种植和消费。褐藻（褐藻门）是富含类藻胶和叶状多糖等多糖类物质的丰富来源。在本研究中，褐藻类富含的类藻胶和叶状多糖在PP（扁波藻）（4.36%）和STM（边缘枝藻）（2.32%）中分别得到高收率。类藻胶和叶状多糖的碳水化合物含量分别为86.91%和87.36%，而叶状多糖的硫酸盐含量为20.68%。PP中的类藻胶主要由葡萄糖和甘露糖组成，然而STM中的叶状多糖主要由岩藻糖、半乳糖和木糖组成。FT-IR下峰表示类藻胶和叶状多糖的碳水化合物，除了1219 cm-1和843 cm-1，这两个峰对应叶状多糖中的硫酸盐基团。类藻胶的分子量为3-5 kDa，叶状多糖的分子量也是2-6 kDa。Fucoidan和Laminarin都对Vero细胞没有细胞毒性。然而，Fucoidan对人肝癌细胞（HepG2）具有细胞毒性（IC50-24.4 ± 1.5 µg/mL）。同时，Laminarin对人结肠癌细胞（HT-29）也显示出细胞毒性（IC50-57 ± 1.2 µg/mL）。AO/EB（橙酸/溴化乙锭）试验表明，Laminarin和Fucoidan对应的处理分别导致细胞凋亡和坏死。DNA断裂结果支持坏死性癌细胞死亡。因此，PP和STM中的Laminarin和Fucoidan是潜在的抗癌治疗的生物活性化合物。© 2023. Springer Nature Limited.
Marine seaweeds are rich source of polysaccharides present in their cell wall and are cultivated and consumed in China, Japan, Korea, and South Asian countries. Brown seaweeds (Phaeophyta) are rich source of polysaccharides such as Laminarin and Fucoidan. In present study, both the laminarin and fucoidan were isolated was yielded higher in PP (Padina pavonica) (4.36%) and STM (Stoechospermum marginatum) (2.32%), respectively. The carbohydrate content in laminarin and fucoidan was 86.91% and 87.36%, whereas the sulphate content in fucoidan was 20.68%. Glucose and mannose were the major monosaccharide units in laminarin (PP), however, fucose, galactose, and xylose in fucoidan (STM). FT-IR down peaks represent the carbohydrate of laminarin and fucoidan except, for 1219 cm-1, and 843 cm-1, illustrating the sulphate groups of fucoidan. The molecular weight of laminarin was 3-5 kDa, and the same for fucoidan was 2-6 kDa, respectively. Both the Fucoidan and Laminarin showed null cytotoxicity on Vero cells. Contrastingly, the fucoidan possess cytotoxic activity on human liver cancer cells (HepG2) (IC50-24.4 ± 1.5 µg/mL). Simultaneously, laminarin also shown cytotoxicity on human colon cancer cells (HT-29) (IC50-57 ± 1.2 µg/mL). The AO/EB (Acriding Orange/Ethidium Bromide) assay significantly resulted in apoptosis and necrosis upon laminarin and fucoidan treatments, respectively. The DNA fragmentation results support necrotic cancer cell death. Therefore, laminarin and fucoidan from PP and STM were potential bioactive compounds for anticancer therapy.© 2023. Springer Nature Limited.