在芯片上开发细胞低温保存方法不仅对生物医学科学至关重要，而且代表了保存传统细胞样本的一种创新方法。本研究提出了一种直接细胞低温冷冻和解冻的简单方法，可实现细胞的长期储存。冷冻过程中，将细胞与传统细胞低温保存溶液一同注入微通道，并使用含有异丙醇的自封袋将芯片封装起来，随后存放于-80℃的冰箱中。解冻过程中，微柱阵列的间距为8微米，被巧妙地纳入到微流控芯片设计中，以有效抑制通道中的细胞。乳腺癌细胞系MDA-MB-231和B47D在低温保存了一个月至一年后成功解冻并生长。这些发现提供了一种在微流控芯片上进行直接细胞低温冷冻和解冻的方法，以用于后续实验。© 2023作者。由日本生命科学、生物技术和农业化学学会代表牛津大学出版。

Developing cell cryo-preservation methods on chips is not only crucial for biomedical science but also represents an innovative approach for preserving traditional cell samples. This study presents a simple method for direct cell freezing and thawing on chip, allowing for long-term storage of cells. During the freezing process, cells were injected into the microchannel along with a conventional cell cryopreservation solution, and the chip was packed using a self-sealing bag containing isopropyl alcohol and then stored in a -80 °C refrigerator until needed. During the thawing process, microcolumn arrays with a spacing of 8 μm were strategically incorporated into the microfluidic chip design to effectively inhibit cells from the channel. The breast cancer cell lines MDA-MB-231 and B47D demonstrated successful thawing and growth after cryopreservation for one month to one year. These findings offer a direct cell freezing and thawing method on a microfluidic chip for subsequent experiments.© The Author(s) 2023. Published by Oxford University Press on behalf of Japan Society for Bioscience, Biotechnology, and Agrochemistry.