多发性硬化症（MS）是一种自身免疫中枢神经系统（CNS）疾病，其特征为脱髓鞘、慢性炎症和神经元破坏。地区性脱髓鞘、炎症反应、疤痕形成以及各种轴突损伤是MS的病理特征。姜黄素是从姜黄植物的根茎中提取的疏水性多酚类化合物。除了抗炎作用外，这种化合物还具有抗氧化、抗癌和神经保护等有益的治疗效果。本研究的目的是探讨姜黄素在治疗实验性自身免疫性脑脊髓炎（EAE）中的潜在益处，该疾病为多发性硬化症动物模型。我们使用雌性C57BL/6小鼠通过神经髓鞘糖蛋白（MOG）诱导来引发EAE。姜黄素100和200毫克/千克的剂量在EAE诱导的第一天开始口服给予治疗组。在EAE诱导后的第25天，从安乐死的动物中提取了大脑和脾细胞。评估了脱髓鞘和白细胞浸润、增殖、细胞因子和基因表达谱。我们的研究结果表明，低剂量和高剂量的姜黄素均减缓了EAE的进展。组织学分析显示白细胞浸润中枢神经系统较轻。姜黄素治疗增强了Th2和Treg细胞的IL-4、IL-10和TGF-β分泌，同时显著降低了IFN-γ和TNF-α。姜黄素诱导了Th2和Treg细胞的细胞因子产生和转录因子基因表达（IL-13、GATA3、STAT6和IL-35、CTLA4、Foxp3），以及抗炎细胞因子（IL-27、IL-33）。总的来说，这些发现提供了额外的证据证明姜黄素通过促进Treg和Th2细胞极化能减缓EAE的疾病发展并缓解症状。他们支持姜黄素在多发性硬化症中的潜在治疗作用。© 2023. 作者（们），在 Springer Nature B.V. 独家授权下。

Multiple sclerosis (MS) is an autoimmune central nervous system (CNS) disorder indicated by demyelination, chronic inflammation, and neuronal destruction. Regional demyelination, inflammation responses, scar development, and various axonal damage are pathological characteristics of MS. Curcumin is a hydrophobic polyphenol extracted from the rhizome of the turmeric plant. In addition to anti-inflammatory effects, beneficial therapeutic effects such as antioxidant, anti-cancer and nerve protection have also been seen from this compound. The purpose of the current investigation was to provide light on the potential benefits of Curcumin in treating experimental autoimmune encephalomyelitis (EAE), the animal model of MS.in Female C57BL/6 mice were used to induce EAE through myelin oligodendroglial glycoprotein (MOG). Curcumin doses of 100 and 200 mg/kg were administered orally in the treatment groups starting on the first day of EAE induction. Brains and splenocytes were extracted from euthanized animals on day 25 following EAE induction. Demyelination and leukocyte infiltration, proliferation, cytokine, and gene expression profiles were assessed. Our findings demonstrate that both low and high doses of Curcumin decreased the progression of EAE. Histological analyses revealed low infiltration of leukocytes into the CNS. Curcumin therapy enhanced Th2 and Treg cell secretion of IL-4, IL-10, and TGF-β although considerably decreasing IFN-γ and TNF-α. Curcumin-induced Th2 and Treg cell cytokine production and transcription factor gene expression (IL-13, GATA3, STAT6 and IL-35, CTLA4, Foxp3) and anti-inflammatory cytokines (IL-27, IL-33).Overall, these findings provide additional evidence that Curcumin can slow disease development and alleviate symptoms in EAE through stimulating Treg and Th2 cell polarization. They support Curcumin's potential therapeutic role in MS.© 2023. The Author(s), under exclusive licence to Springer Nature B.V.