本研究比较了从P.菌丝中提取的葡萄糖和蔗糖喂养产生的六种硫酸化多糖的生物活性。通过预处理RAW264.7细胞中诱导的脂多糖(LPS)引起的炎症来评估这些多糖的抗炎潜力。在所测试的多糖中，以40克/升浓度的蔗糖喂养产生的硫酸化多糖（称为"suc 40"）表现出最高的抗炎活性，达到83%，对IL-6和TNF-α的分泌抑制率分别为33%。它通过抑制p-38和c-Jun N末端激酶(JNK) MAPK信号通路来实现这一效果。另一方面，以20克/升浓度的葡萄糖喂养产生的硫酸化多糖（称为"glc 20"）表现出最强的抗肺癌活性。这是通过诱导与凋亡相关的分子，如聚(ADP-核糖)聚合酶 (PARP) 和 CHOP 来实现的。此外，与其他所测试的多糖相比，glc 20具有最高的硫酸盐、岩藻糖和甘露糖含量。这表明单糖残基的组成是影响这些硫酸化多糖的抗炎和抗癌活性的关键因素。总的来说，本研究凸显了从P.菌丝中提取的硫酸化多糖作为具有抗炎和抗癌特性的生物活性化合物的潜力。版权所有© 2023。由Elsevier B.V.出版。

This study compares the bioactivity of six sulfated polysaccharides derived from glucose- and sucrose-feeding extracted from P. cocos. Anti-inflammatory potentials of these polysaccharides were evaluated by pretreating lipopolysaccharide (LPS)-induced inflammation in RAW264.7 cells. Of the tested polysaccharides, the sulfated polysaccharide derived from sucrose-feeding at the concentration of 40 g/l (referred to as "suc 40") exhibited the highest anti-inflammatory activity, of 83 %, and 33 % inhibition of IL-6 and TNF-α secretion, respetively. It achieved this by inhibiting the p-38 and c-Jun N-terminal kinase (JNK) MAPK signaling pathways. On the other hand, the sulfated polysaccharide derived from glucose-feeding at a concentration of 20 g/l (referred to as "glc 20") demonstrated the greatest anti-lung cancer activity. This was achieved by inducing apoptotic-related molecules, such as poly (ADP-ribose) polymerase (PARP) and CHOP. Furthermore, glc 20 had the highest contents of sulfate, fucose, and mannose compared to the other tested polysaccharides. This suggests that the composition of monosaccharide residues are critical factors influencing the anti-inflammatory and anti-cancer activities of these sulfated polysaccharides. Overall, this study highlights the potential of sulfated polysaccharides derived from P. cocos to function as bioactive compounds with anti-inflammatory and anti-cancer properties.Copyright © 2023. Published by Elsevier B.V.