HSP60是热休克蛋白（HSP）家族成员，有助于线粒体蛋白正确折叠。Survivin是凋亡抑制蛋白家族成员之一，对癌症的进展起到重要作用。它们能够形成HSP60-survivin复合物，并参与各种肿瘤的发展。癌症基因组图谱（TCGA）数据库显示，HSP60和survivin与OSCC患者的mRNA表达水平相关。此外，利用免疫组织化学在组织微阵列（TMA）中研究了OSCC和相邻非癌性鳞状上皮组织（Non-CCSE）中HSP60和survivin蛋白的表达。与正常组织相比，通过全癌分析发现HSP60和survivin在大多数癌症中的水平明显增加。在mRNA和蛋白水平上，与Non-CCSE相比，OSCC样本中HSP60和survivin的表达显著增加（两者的P ＜ 0.05）。此外，表达上调的HSP60在mRNA和蛋白表达水平上与survivin显示出正相关（所有P ＜ 0.001）。OSCC患者的临床分期进展较快或存在淋巴结转移（LNM）的患者表达HSP60水平较高（P = 0.004，P = 0.006）。在多变量生存分析中，蛋白质HSP60和survivin的高水平与OSCC患者的总生存率显著呈负相关（P = 0.018，P = 0.040）。根据上述结果，HSP60和survivin的过表达可能作为独立的生物标志物，预测OSCC的不良预后。高水平的HSP60和survivin可能作为术后切除的OSCC患者的新的不良预后生物标志物。© 2023. BioMed Central Ltd., Springer Nature的一部分。

HSP60 is a heat shock proteins (HSPs) family member and help mitochondrial protein to fold correctly. Survivin is one of the inhibitors of apoptosis protein family member, which plays a significant part in cancer progression. They were capable of forming HSP60-survivin complexes and involved in the development of various tumors.The Cancer Genome Atlas (TCGA) database demonstrated that HSP60 and survivin and their correlation on mRNA expression level with OSCC patients. Besides, expression of HSP60 and survivin proteins was studied utilizing immunohistochemistry in tissue microarrays (TMA) in OSCC and in adjacent non-cancerous squamous epithelium (Non-CCSE) tissues.Significantly increased levels of HSP60 and survivin in most cancers compared to normal tissue by pan-cancer analysis. HSP60 and survivin proved a significantly increased expression in OSCC samples compared to Non-CCSE both on mRNA and protein (both P < 0.05). Additionally, elevated HSP60 displayed a positive correlation with survivin in terms of mRNA and protein expression levels (all P < 0.001). Patients with OSCC who had advanced clinical stage or lymph node metastasis (LNM) showed higher HSP60 expression (P = 0.004, P = 0.006, respectively). Higher levels of the proteins HSP60 and survivin were significantly inversely correlated relationship with OSCC patients' overall survival rates in multivariate survival analysis (P = 0.018, P = 0.040). From the above results, overexpression of HSP60 and survivin protein may serve as independent biomarkers predicting poor prognosis in OSCC.Elevated HSP60 and survivin might be served as novel poor prognosis biomarkers for surgically resected OSCC patients.© 2023. BioMed Central Ltd., part of Springer Nature.