骨肉瘤是最常见的原发性恶性骨肿瘤，具有高转移和复发的风险。代谢重编程是骨肉瘤和其他肿瘤的一个标志性特征，与遗传和表观遗传改变相关。RUNX2是骨母细胞分化的重要转录因子，基因的异常表达有助于骨肉瘤的发展和进展。为了确定RUNX2沉默对骨肉瘤转录组和代谢组学特征的影响，我们生成了稳定表达RUNX2 shRNA的SJSA-1骨肉瘤细胞和稳定表达混乱shRNA的SJSA-1细胞，并使用Illumina NovaSeq 6000和超高效液相色谱-飞行时间质谱联用技术分析了这两种细胞类型的转录组和代谢组学特征。研究人员可以使用这些数据集来鉴定RUNX2的新靶点，并阐明RUNX2在骨肉瘤发生机制和代谢重编程中的作用和潜在机制。© 2023 The Author(s).

Osteosarcoma is the most common primary malignant bone tumor with a high risk of metastasis and recurrence. Metabolic reprogramming is a hallmark of osteosarcoma and other cancers and is associated with genetic and epigenetic alterations. RUNX2 is an important transcription factor for osteoblastic differentiation, and aberrant expression of the gene contributes to the development and progression of osteosarcoma. To identify the effects of RUNX2 silencing on transcriptomic and metabolomic profiles in osteosarcomas, we generated SJSA-1 osteosarcoma cells stably expressing RUNX2 shRNA and SJSA-1 cells stably expressing scramble shRNA and analyzed transcriptome and metabolome profiles in the two cell types using Illumina NovaSeq 6000 and ultrahigh-performance liquid chromatography coupled with time-of-flight mass spectrometry, respectively. The datasets can be used by researchers to identify novel targets of RUNX2 and elucidate the role and underlying mechanism of RUNX2 in osteosarcoma pathogenesis and metabolic reprogramming.© 2023 The Author(s).