五味子果油的活性成分及其对丙酸杆菌诱导的HaCaT细胞炎症的影响。
Active Ingredients of Schisandra chinensis Fruit Oil and their Effect on Propionibacterium acnes-Induced Inflammation in HaCaT Cells.
发表日期：2023 Aug 23
作者：
Pingping Lv, Huirong Zhang, Meiling Tai, Biao Che, Dan Yu, Ying Zhang, Haodong Li, Xianyao Meng, Miaomiao Guo, Li Li
来源：
ANTIOXIDANTS & REDOX SIGNALING
摘要：
丙酸杆菌可引起促炎因子（如环氧酶-2，前列腺素E2，白细胞介素-1β和肿瘤坏死因子-α）的上调，增加活性氧（ROS）水平和钙离子内流。这导致抗菌肽LL-37水平升高，皮肤发炎，出现红肿、瘙痒等症状。五味子果油（SCO）含有各种抗氧化和抗炎成分的木脂素活性成分。本研究通过超临界CO2流体萃取得到SCO，利用丙酸杆菌诱导的HaCaT细胞模型研究了SCO的抗炎作用。建立并验证了基于反相高压液相色谱-二极管阵列检测器的方法，用于同时检测五种木脂素成分。通过ELISA试剂盒和免疫印迹法测定了促炎因子和LL-37的水平，并通过流式细胞术检测Ca2+和ROS水平。实验结果显示，schisanol A、schisanol B、schisanin A、schisanin B和schisanin C的含量分别为33.89 ± 0.24、14.89 ± 0.45、8.92 ± 0.02、29.14 ± 0.67和4.74 ± 0.09 mg/g。研究表明，SCO可以通过抑制COX-2/PGE2和NF-κB信号通路来缓解皮肤炎症。此外，SCO能抑制ROS产生，显著阻断Ca2+内流，缓解细胞损伤，调节抗菌肽LL-37的含量。总之，我们的研究阐明了SCO在细胞模型中的抗炎活性，并为其作为皮肤护理原料的应用提供了科学依据。© 2023 The Author(s). Published by IMR Press.
Propionibacterium acnes causes upregulation of inflammatory factors, such as cycloxygenase-2, prostaglandin E2, interleukin-1β, and tumor necrosis factor-alpha, increased levels of reactive oxygen species (ROS) and inward flow of calcium ions. This causes increased levels of the antimicrobial peptide LL-37 and inflammation of the skin, leading to redness, swelling, itching and other symptoms. Schisandra chinensis fruit oil (SCO) is rich in lignan active ingredients with various antioxidant and anti-inflammatory properties.In this study, SCO is obtained by supercritical CO2 fluid extraction. SCO's anti-inflammatory actions were investigated using P. acnes-induced inflammation HaCaT cells model. A method based on reversed-phase high-pressure liquid chromatography with a diode array detector was developed and validated for the simultaneous detection of five lignan components. Levels of inflammatory factors and LL-37 were measured by ELISA kit and western blot respectively. Ca2+ and ROS levels detected by flow cytometry.The experimental results show that the contents of schisanol A, schisanol B, schisanin A, schisanin B, and schisanin C were 33.89 ± 0.24, 14.89 ± 0.45, 8.92 ± 0.02, 29.14 ± 0.67, and 4.74 ± 0.09 mg/g, respectively. Studies have demonstrated that SCO can alleviate skin inflammation by inhibiting the COX-2/PGE2 and NF-κB signalling pathway. In addition, SCO can inhibit ROS production, significantly block inward Ca2+ flow, alleviate cell damage, and modulate the content of the antimicrobial peptide LL-37.In summary, our study elucidated the anti-inflammatory activity of SCO in a cell model and provided a scientific basis for its application as a raw material in skin care.© 2023 The Author(s). Published by IMR Press.