伊南乌榄叶提取物中发现了两种新的2-芳基苯并[b]呋喃类化合物,并研究了它们对肝细胞癌的抗癌和抗氧化作用。
Two new 2-arylbenzo[b]furans from Itea indochinensis and their anti-hepatocellular carcinoma and anti-oxidant effects.
发表日期:2023 Sep 04
作者:
Fang-Fang Xu, Jin-Zhen Jian, Yi-Jv Li, Zhi-Wei Wang, Guo-Yong Luo, Wu-De Yang
来源:
ANTIOXIDANTS & REDOX SIGNALING
摘要:
首次从越南瑞香(Itea indochinensis)的枝叶的95%乙醇提取物中鉴定出两种新的2-芳基苯并[b]呋喃类化合物(1-2)和十种已知化合物(3-12)。主要通过UV、IR、1D/2D NMR和HRMS光谱的广泛分析,确定了它们的结构。MTT细胞增殖抑制实验的结果表明,化合物1具有良好的选择性,对SK-HEP-1细胞的增殖有中度抑制作用,IC50值为22.3μM;对HepG2细胞的抑制作用较弱,20μM时抑制率为25%;对A549细胞的增殖几乎没有明显抑制作用(20μM)。另外,化合物1表现出对ABTS·+自由基的显著清除能力,IC50值为0.11mg/mL;对DPPH和O2·-自由基的清除效果较弱,浓度为1mg/mL时清除率分别为32.93%和21.49%。
Two new 2-arylbenzo[b]furans (1-2) and ten known compounds (3-12) were identified from the 95% EtOH extract of the branches and leaves of Itea indochinensis for the first time. Their structures were determined mainly based on extensive analyses of UV, IR, 1D/2D NMR and HRMS spectra. The results of MTT assays demonstrated the anti-tumor potential of compound 1 with good selectivity, which displayed moderate inhibitory effects on proliferation of SK-hep-1 cells with IC50 value of 22.3 μM, while weak inhibitory effect on proliferation of HepG2 cells with an inhibition rate of 25% at 20 μM, and no obviously inhibitory effect on proliferation of A549 cells at 20 μM. In addition, compound 1 exhibited its significant scavenging capacity on ABTS·+ free radical with an IC50 value of 0.11 mg/mL, while weak scavenging effects on DPPH and O2·- radicals with scavenging ratios of 32.93% and 21.49% at 1 mg/mL, respectively.