一种用于基于质谱的蛋白质组学的尿液细胞外囊泡分离的经过验证且可应用于临床的样品制备方案。
A Robust and Clinically Applicable Sample Preparation Protocol for Urinary Extracellular Vesicle Isolation Suitable for Mass Spectrometry-Based Proteomics.
发表日期：2023
作者：
Leyla A Erozenci, Irene V Bijnsdorp, Sander R Piersma, Connie R Jimenez
来源：
Protein & Cell
摘要：
尿液胞外囊泡（uEVs）是非侵入性蛋白质生物标志物的丰富来源。然而，为了将其转化为临床应用，需要一种易于使用且与蛋白质组学兼容的uEVs分离方案。在这里，我们提供了一个快速且可应用于临床的uEVs分离方案的详细描述。我们侧重于分离程序以及随后使用LC-MS/MS技术进行的深入蛋白质组学表征。以前列腺癌患者的尿液样本和对照组尿液样本为例，展示了如何进行差异分析。© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
Urinary extracellular vesicles (uEVs) are a rich source of noninvasive protein biomarkers. However, for translation to clinical applications, an easy-to-use uEV isolation protocol is needed that is compatible with proteomics. Here, we provide a detailed description of a quick and clinical applicable uEV isolation protocol. We focus on the isolation procedure and subsequent in-depth proteome characterization using LC-MS/MS-based proteomics. As an example, we show how differential analyses can be performed using urine samples obtained from prostate cancer patients, compared to urine from controls.© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.