为了评估钇铝石榴石（YAG）激光玻璃体松解术对眼内组织的安全性。本试验选取了36只新西兰兔，分为如下几组：1000组（n=12），采用1000mJ（5mJ×200次射频）的YAG激光治疗；2000组（n=12），采用2000mJ（5mJ×400次射频）的YAG激光治疗；3000组（n=12），采用3000mJ（5mJ×600次射频）的YAG激光治疗。在实验组中，选择其中一只眼睛作为研究眼，而另一只眼睛未经处理作为对照组。采用眼内压（IOP）、裂隙灯检查、光学相干断层扫描（OCT）、透射电子显微镜（TEM）以及淀粉酶的水悬液炎症细胞因子（白细胞介素-1α（IL-1α）、白细胞介素-1β（IL-1β）、白细胞介素-8（IL-8）和肿瘤坏死因子-α（TNF-α））等方式对兔子进行检查。实验组的IOP、裂隙灯检查和OCT结果均未发现异常。TEM检查3000组显示：第1天出现中性粒细胞和线粒体肿胀，第14天出现成纤维细胞和新型胶原。1000组和2000组的TEM检查结果未发现异常。IL-1α和TNF-α水平在12小时内升高，并在第3天恢复至基线水平。IL-1β水平在12小时内升高，并在第7天恢复至基线水平。IL-8水平在第1天升高，并在第3天恢复至基线水平。距离破坏点到晶状体和视网膜的距离超过2mm，单次治疗的总能量不超过2000mJ时，YAG激光玻璃体松解术是安全的。©2023 作者。

To evaluate the safety of yttrium-aluminum-garnet (YAG) laser vitreolysis for intraocular tissues.Thirty-six New Zealand rabbits were divided as follows: Group 1000 (n = 12) treated with YAG laser of 1000 mJ (5 mJ × 200 shots), Group 2000 (n = 12) treated with YAG laser of 2000 mJ (5 mJ × 400 shots), Group 3000 (n = 12) treated with YAG laser of 3000 mJ (5 mJ × 600 shots). Either a single eye was chosen as the study eye in study groups while the other was untreated as the control group. Intraocular pressure (IOP), slit-lamp, optical coherence tomography (OCT), transmission electron microscopy (TEM), and inflammatory cytokines of aqueous humor (interleukin-1α (IL-1α), interleukin-1β (IL-1β), interleukin-8 (IL-8), and tumor necrosis factor-α (TNF-α)) were performed to examine the rabbits.There were no abnormalities in the study groups of IOP, slit-lamp, and OCT examinations. Group 3000 of TEM showed: neutrophils and mitochondrial swelling on day 1, and fibroblasts and neocollagen on day 14. No abnormalities were observed in Group 1000 and 2000 of TEM. Levels of IL-1α and TNF-α increased at 12 h and decreased to baseline on day 3. Levels of IL-1β increased at 12 h and decreased to baseline on day 7. Levels of IL-8 increased on day 1 and decreased to baseline on day 3.YAG laser vitreolysis is safe when the distance is more than 2 mm from ablation point to the lens and the retina, and the total energy is less than 2000 mJ for one treatment procedure.© 2023. The Author(s).