癌症，特别是肺癌（LC），的主要特征之一是异常细胞分裂。在早期恶性病变和恶性病变中均观察到Kinesin家族成员C1（KIFC1 / HSET）的异常表达，该成员参与有丝分裂细胞分裂并确保在分裂过程中染色体的赤道对齐。文献中没有研究探讨KIFC1在肺癌的诊断和随访中的作用。本研究旨在探讨KIFC1在不同组织学亚型诊断的肺癌患者中的表观遗传角色，包括诊断、分期和预后。在39例肺癌患者的肿瘤、配对正常组织和血液样品中，采用DNA/RNA分离，检查了KIFC1基因的表达和甲基化状态，并采用39例健康对照组血液样品。在RNA分离后，通过定量实时荧光PCR（qRT-PCR）方法进行cDNA合成，检测了KIFC1基因的表达变化。采用甲基化特异性PCR（MSP）方法确定了KIFC1基因的甲基化状态。本研究采用统计方法分析了KIFC1基因的表达/甲基化特征以及患者的临床病理特征。检测到62.1%的患者组织中95.8%的组织发生KIFC1基因表达升高的低甲基化。与配对正常组织相比，患者肿瘤组织中KIFC1基因表达水平增加了3.2倍，血清中KIFC1基因表达水平增加了2.4倍。对患者的临床参数、甲基化和表达结果进行统计比较，发现KIFC1表达与肺癌转移、肿瘤分期和肿瘤分级之间存在统计学显著性。综上所述，与健康人群相比，肺癌患者中KIFC1基因表达水平的增加更高。因此，由于低甲基化引起的KIFC1基因表达水平的增加可作为肺癌的筛查生物标志物。此外，KIFC1基因的甲基化特征可能是确定肺鳞状细胞癌亚型的潜在生物标志物。该研究结果需要用更多患者进行分析和延续。Copyright © 2023 Elsevier GmbH. All rights reserved.

One of the main features of cancer, especially lung cancer (LC), is abnormal cell division. Abnormal expression of kinesin family member C1 (KIFC1/HSET), which is involved in mitotic cell division and ensures equatorial alignment of chromosomes during division, is observed in both premalignant and malignant lesions. There are no studies in the literature addressing the role of KIFC1 in the diagnosis and follow-up of LC. In this study, we investigated the epigenetic role of KIFC1 in the diagnosis, stage, and prognosis of various histological subtypes diagnosed with LC.The expression and methylation status of the KIFC1 gene were examined after DNA/RNA isolation in tumor, conjugate normal tissue, and blood samples from 39 patients diagnosed with LC and in blood samples from 39 healthy controls. Changes in KIFC1 gene expression were examined by the Quantitative Real Time-PCR (qRT-PCR) method after cDNA synthesis following RNA isolation. The Methylation-Specific PCR (MSP) method was used to determine the methylation status of the KIFC1 gene. In this study, the expression/methylation profiles of the KIFC1 gene and the clinical and pathological characteristics of the patients were analyzed by statistical methods.Hypomethylation was detected in 95.8% of the 62.1% of patients' tissues with increased KIFC1 gene expression. The expression level of the KIFC1 gene was found to be increased 3.2-fold in the tumor tissues of the patients compared with the conjugated normal tissues and 2.4-fold in the serum of the patients compared with the healthy serum. Statistical comparison of patients' clinical parameters and methylation and expression results revealed statistical significance between KIFC1 expression and metastasis, tumor stage and tumor grade.In conclusion, the increase in the expression level of the KIFC1 gene is higher in patients diagnosed with LC than in the healthy population, and therefore, the increase in the expression level of the KIFC1 gene due to hypomethylation can be used as a screening biomarker in LC. It can also be considered that the methylation profile of the KIFC1 gene may be a potential biomarker for determining the subtype of squamous cell carcinoma in LC. The results of the study need to be analyzed and continued with a larger number of patients.Copyright © 2023 Elsevier GmbH. All rights reserved.