最近几年，胞内寄生虫弓形虫（Toxoplasma gondii）表现出了抗药性和治疗失败的现象。Dimedone（DIM）作为一种具有抗菌和抗癌特性的新化学化合物已被引入。本研究旨在研究DIM纳米颗粒在弓形虫动物模型中的潜在保护作用。通过使用MTT对Vero细胞系进行DIM的细胞毒性评估，通过与对照组比较计数弓形虫数量来评估DIM对Toxoplasma gondii的影响。利用苏木精-伊红染色检查动物模型的肝细胞中寄生虫的发病率和毒力。此外，比较不同组别小鼠肝组织中表示氧化应激的各项指标。纳米颗粒形式的释放时间明显长于自由药物。Nano-DIM的IC50为60 µM，并且Nano-DIM和Nano-PYR（纳米原磷胺）组中细胞内寄生虫增殖的减少程度明显低于自由药物的体外实验。用Dimedone纳米医药处理的组的组织病理学检查表明，与弓形虫组相比，肝中央静脉上皮的解体程度和肝细胞的浸润和液泡化程度较低。此外，与其他组相比，纳米处理组的某些氧化应激指标的水平较低。本研究结果表明，DIM可以作为一种具有抗弓形虫活性的有前景的化合物，并可以防止其在细胞中的增殖。版权所有© 2023 The Authors. 由Elsevier Masson SAS出版。保留所有权利。

Toxoplasma gondii, an intracellular parasite, has shown drug resistance and therapeutic failure in recent years. Dimedone (DIM) has been introduced as a new chemical compound with anti-bacterial and anti-cancer properties. The aim of this study was to investigate the potential protective role of DIM nanoparticles in an animal model of toxoplasmosis. Cytotoxicity of DIM on Vero cell line assessed using MTT, and the effect of DIM on Toxoplasma gondii was evaluated by counting the number of parasites compared to the control group in vitro. The rate of pathogenesis and virulence of the parasite was checked on the liver cells of the animal model using hematoxylin-eosin staining. Furthermore, various parameters indicating oxidative stress were compared in mouse liver tissue in different groups. The release of the nanoparticle form was significantly longer than the free drugs. The IC50 of Nano-DIM was 60 µM and the reduction of intracellular parasite proliferation in the group Nano-DIM and Nano-PYR (Nano-primethamine) was significantly lower than the free drugs in vitro. Histopathology examination in the groups treated with dimedone nanomedicine showed that the degree of disintegration of the epithelium of the central vein of the liver and infiltration and vacuolization of liver cells were lower compared to the toxoplasmosis group. Additionally, the level of some oxidative stress indicators was observed to be lower in the nano-treated groups compared to other groups. The results of this study showed DIM can be used as a promising compound for anti-T. gondii activity and can prevent the proliferation of it in cells.Copyright © 2023 The Authors. Published by Elsevier Masson SAS.. All rights reserved.