粟斑螟（Spodoptera frugiperda，FAW）是一种入侵性多食性鳞翅目害虫，它通过解毒酶来消除其饮食中遇到的毒性化合物，包括杀虫剂。尽管我们已经了解了其解毒酶库存，但适应不同毒性化合物的机制仍然未被充分探索。我们使用 Sf9 细胞研究了转录因子 Cap n' collar isoform C（CncC）和肌腱腱膜纤维肉瘤（Maf）在调节解毒反应中的作用。我们过表达了 CncC、Maf 或两个基因，并敲除了（KO）CncC 或其抑制子 Kelch-like ECH associated protein 1（Keap1）。只有 CncC 和 Maf 的联合过表达才能赋予对芸香碱（I3C）和溴仿（methoprene）增加的耐受性。这两种分子会在不同细胞系中诱导产生活性氧种（ROS）脉冲。使用抗氧化剂可以逆转 ROS 脉冲并恢复对 I3C 和溴仿的耐受性。解毒酶活性根据细胞系不同而有所变化。抑制 Keap1 显著增加了细胞色素 P450、羧酯酶和谷胱甘肽 S-转移酶的活性。RNAseq 实验表明，CncC 主要调节解毒基因的表达，同时处于多个信号通路（生殖和免疫）的交汇点，维持稳态。我们在 Sf9 细胞系中提供了新的数据，表明 CncC:Maf 途径在 FAW 对天然和合成异物的响应中起着中心作用。这些知识有助于更好地理解解毒基因表达，并可能有助于设计下一代害虫控制措施。版权所有 © 2023 Elsevier Inc. 保留所有权利。

Spodoptera frugiperda (fall armyworm, FAW) is an invasive polyphagous lepidopteran pest that has developed sophisticated resistance mechanisms involving detoxification enzymes to eliminate toxic compounds it encounters in its diet including insecticides. Although its inventory of detoxification enzymes is known, the mechanisms that enable an adapted response depending on the toxic compound remain largely unexplored. Sf9 cells were used to investigate the role of the transcription factors, Cap n' collar isoform C (CncC) and musculoaponeurotic fibrosarcoma (Maf) in the regulation of the detoxification response. We overexpressed CncC, Maf or both genes, and knocked out (KO) CncC or its repressor Kelch-like ECH associated protein 1 (Keap1). Joint overexpression of CncC and Maf is required to confer increased tolerance to indole 3-carbinol (I3C), a plant secondary metabolite, and to methoprene, an insecticide. Both molecules induce reactive oxygen species (ROS) pulses in the different cell lines. The use of an antioxidant reversed ROS pulses and restored the tolerance to I3C and methoprene. The activity of detoxification enzymes varied according to the cell line. Suppression of Keap1 significantly increased the activity of cytochrome P450s, carboxylesterases and glutathione S-transferases. RNAseq experiments showed that CncC mainly regulates the expression of detoxification genes but is also at the crossroads of several signaling pathways (reproduction and immunity) maintaining homeostasis. We present new data in Sf9 cell lines suggesting that the CncC:Maf pathway plays a central role in FAW response to natural and synthetic xenobiotics. This knowledge helps to better understand detoxification gene expression and may help to design next-generation pest insect control measures.Copyright © 2023 Elsevier Inc. All rights reserved.