癌症驱动基因（CDGs）和驱动突变扰乱了许多关键细胞活动的稳态，从而在肿瘤的发生和发展中起到了关键作用。本研究基于一系列在线数据库进行了综合生物信息学分析，旨在鉴定头颈癌中突变频率较高的驱动基因。高频的Multiple Myeloma Overexpressed（MYEOV）遗传变异频率和表达水平与头颈癌患者的预后较差相关。在头颈肿瘤样本和细胞中，MYEOV被显著上调。一致地，MYEOV过表达极大地增强了头颈癌细胞的侵袭性，通过促进集落形成、细胞侵袭和细胞迁移。相反，MYEOV沉默减弱了癌细胞的侵袭性，并抑制了口腔正置移植瘤模型中的肿瘤生长和转移。总之，MYEOV在头颈癌中高表达，较高的突变频率与头颈癌患者的预后较差相关。MYEOV在头颈癌中作为一个癌基因，通过促进肿瘤细胞集落形成、侵袭和迁移以及促进口腔正置移植瘤模型的肿瘤生长和转移发挥作用。© 2023. 作者，保留Springer Science + Business Media，LLC的独家许可权，属于Springer Nature的一部分。

Cancer driver genes (CDGs) and the driver mutations disrupt the homeostasis of numerous critical cell activities, thereby playing a critical role in tumor initiation and progression. In this study, integrative bioinformatics analyses were performed based on a series of online databases, aiming to identify driver genes with high frequencies of mutations in head and neck cancers. Higher myeloma overexpressed (MYEOV) genetic variation frequency and expression level were connected to a poorer prognosis in head and neck cancer patients. MYEOV was dramatically upregulated within head and neck tumor samples and cells. Consistently, MYEOV overexpression remarkably enhanced the aggressiveness of head and neck cancer cells by promoting colony formation, cell invasion, and cell migration. Conversely, MYEOV knockdown attenuated cancer cell aggressiveness and inhibited tumor growth and metastasis in the oral orthotopic tumor model. In conclusion, MYEOV is overexpressed in head and neck cancer, with greater mutation frequencies correlating to a poorer prognosis in head and neck cancer patients. MYEOV serves as an oncogene in head and neck cancer through the promotion of tumor cell colony formation, invasion, and migration, as well as promoting tumor growth and metastasis in the oral orthotopic tumor model.© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.