我们进行了一项生化和组织学评估，研究黄连素在环磷酰胺诱导的早发性卵巢衰竭（POF）模型中是否具有保护和/或治疗作用。我们将28只雌性Wistar白化种大鼠分为4组[对照组、POF组、环磷酰胺（CP）+黄连素（Bb）组和POF + Bb组]。POF模型通过腹腔注射50 mg/kg CP第1天后，连续14天每天注射8 mg/kg CP溶解于生理盐水来建立。CP + Bb组与CP一起连续两周接受Bb。POF + Bb组在CP注射结束后连续两周接受黄连素。左卵巢用于病理学评估，右卵巢用于生化分析（组织中肿瘤坏死因子-α（TNF-α），白细胞介素（IL）-1，IL-6水平）。POF组的卵巢损伤评分显著高于其他组（p<0.005）。在POF组中，原始卵泡和初级卵泡计数最低，而次级卵泡和体黄体计数最高（p<0.005）。其他组之间没有显著差异。POF组的生化结果中TNF-α，IL-1和IL-6水平明显升高（p<0.005）。我们证明黄连素通过降低促炎细胞因子可以有效预防和治疗POF。我们相信我们的研究对POF的预防和/或治疗领域将做出可观的贡献。

We conducted a biochemical and histological evaluation of whether berberine has a protective and/or therapeutic effect in a cyclophosphamide-induced premature ovarian failure (POF) model.We divided 28 Wistar albino female rats into 4 groups [control group, POF group, cyclophosphamide (CP)+berberine (Bb) group, and POF+Bb group]. The POF model was established by intraperitoneal administration of 50 mg/kg CP on day 1 followed by 8 mg/kg/day CP dissolved in saline for the following 14 days. The CP+Bb group received Bb concurrently for two weeks with CP. The POF+Bb group received berberine for two weeks following the completion of CP administration. Left ovaries were used for histopathological evaluation and right ovaries were used for biochemical analysis [tumor necrosis factor-alpha (TNF-α), interleukin (IL)-1, IL-6 levels in tissue].Ovarian damage scoring was significantly higher in the POF group than in the other groups (p<0.005). In the POF group, primordial and primary follicle counts were the lowest, while secondary and corpus luteum counts were the highest (p<0.005). There was no significant difference between the other groups. The POF group had significantly elevated levels of TNF-α, IL-1, and IL-6 in the biochemistry results (p<0.005).We demonstrated that berberine could be effective in the protection and treatment of POF by reducing proinflammatory cytokines. We believe that our study can make a considerable contribution to the literature in terms of POF protection and/or treatment.