转基因表达基因是斑马鱼癌症建模的主要方法。传统转基因技术依赖于对单细胞胚胎的注射，但理想情况下，这些转基因应该仅在成年体细胞组织中表达。我们提供了一种在成年斑马鱼中模拟癌症的方法，该方法可以通过电穿孔表达转基因。以黑色素瘤为例，我们演示了表达癌基因，如BRAFV600E以及肿瘤抑制基因PTEN的CRISPR/Cas9灭活的可行性。这些方法可以在任何基因背景中执行，如现有荧光素报告基因系列或casper系列。这些方法可以轻松扩展到其他细胞类型中，以快速进行斑马鱼成年癌症建模。© 2024。作者以独家许可授予 Springer Science+Business Media, LLC，属于 Springer Nature的一部分。

Transgenic expression of genes is a mainstay of cancer modeling in zebrafish. Traditional transgenic techniques rely upon injection into one-cell embryos, but ideally these transgenes would be expressed only in adult somatic tissues. We provide a method to model cancer in adult zebrafish in which transgenes can be expressed via electroporation. Using melanoma as an example, we demonstrate the feasibility of expressing oncogenes such as BRAFV600E as well as CRISPR/Cas9 inactivation of tumor suppressors such as PTEN. These approaches can be performed in any genetic background such as existing fluorophore reporter lines or the casper line. These methods can readily be extended to other cell types allowing for rapid adult modeling of cancer in zebrafish.© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.