人类乳头瘤病毒（HPV）感染与宫颈癌密切相关，尤其是高风险HPV（HR-HPV）基因型的持续感染。因此，对大规模人群进行HPV感染率、年龄特定性、基因型分布以及COVID-19大流行对其影响的调查对HPV筛查和优化疫苗接种至关重要。本研究为横断面研究。我们收集了2018年1月至2022年12月威海市的38056份宫颈上皮细胞标本。根据参与者年龄将研究分为7个年龄组。我们使用一种商业试剂盒进行HPV基因型检测，该试剂盒设计用于检测23种HPV基因型。共有8998名女性感染HPV，总体阳性率为23.64%（8998/38056）。不同年龄组中单一型HPV感染占主导地位，占总感染数的71.33%。最常见的基因型是HR-HPV 16（4.33%），其次是52、58、53和68。在6个低风险HPV基因型中，低风险HPV 42显示了最高的流行率（2.19%），这是一个新的发现。不同年龄组间的流行性差异显著（p<0.01），25岁以下年龄组的感染率最高。在为期3年的COVID-19爆发期间，2020年、2021年和2022年收到的HPV样本数量分别减少了24.03%、14.79%和24.76%。在2018年至2022年期间，年患病率在21.09%至25.30%之间波动，呈下降趋势，而HR-HPV 39、56、31和LR-HPV 42的患病率却上升。本研究表明，该地区存在高HPV感染率和年龄特定性分布特征，以及COVID-19爆发对HPV患病率的影响分析，为该地区控制和预防HPV感染提供了流行病学依据。© 作者（或其雇主）2023年。根据CC BY-NC许可进行再利用。不允许商业再利用。BMJ出版。

Human papillomavirus (HPV) infection is closely associated with cervical cancer, especially the persistent infection of high-risk HPV (HR-HPV) genotypes. Therefore, investigating the HPV prevalence, age-specific, genotype distribution and the impact of the COVID-19 pandemic among large populations was essential for HPV screening and optimising vaccination.This was a cross-sectional study.A total of 38 056 cervical epithelial cell specimens were collected in Weifang city from January 2018 to December 2022. The study was divided into seven age groups based on the age of the participants. HPV genotype testing was performed by using a commercial kit which is designed for the detection of 23 HPV genotypes.A total of 8998 women were infected with HPV, with an overall positive rate of 23.64% (8998/38 056). Single infection of HPV was dominant among different age groups, which accounted for 71.33% of total infections. The most prevalent genotype was HR-HPV 16 (4.33%), followed by 52, 58, 53 and 68. Low-risk HPV (LR-HPV) 42 exhibited the highest prevalence (2.19%) among six LR-HPV genotypes, representing a novel finding. There was a significant difference in the prevalence across different age groups (p<0.01), with the highest prevalence in the group under 25 years old. During the 3 year COVID-19 breakout period, the number of HPV samples received in 2020, 2021 and 2022 was reduced by 24.03%, 14.79% and 24.76%, respectively. In 2018-2022, the annual prevalence varied between 21.09% and 25.30%, with a decreasing trend, while the prevalence of HR-HPV 39, 56, 31 and LR-HPV 42 increased.This study indicates a high-HPV infection rate and age-specific distribution characteristics of HPV genotype infections, as well as analyses of the impact of the COVID-19 outbreak on the HPV prevalence, which provides an epidemiological basis for the control and prevention of HPV infection in this region.© Author(s) (or their employer(s)) 2023. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.