成功从海藻源内生真菌Chaetomium globosum 2020HZ23中获得了两种新型的氯化和氮化异杂黄酮。它们分别为N-丁基-2-aza-2-脱氧chaetoviridin A (1)和N-己基-2-aza-2-脱氧chaetoviridin A (2)。同时，还成功获得了之前已鉴定的chaetoviridin A (3)类似物。这些新代谢物的平面结构以及绝对构型是通过采用谱学技术（1D/2D NMR和HRESIMS）和密度泛函理论（DFT）计算的协同方法来确定的。每个化合物都在体外对A549癌细胞系进行了细胞毒性评估。化合物1和2都展示了显著的细胞毒性，其IC50值分别为13.6和17.5 μM。此外，化合物1和2表现出强大的细胞迁移抑制作用，随着剂量浓度的增加而增强。相比之下，化合物3相对于1和2表现出较低的细胞毒性活性，这表明通过在C-2位置进行N取代和引入侧链可以提高细胞毒性的效力。这一发现可能对设计和改进此类领先化合物的未来研究提供启示。版权所有© 2023 Gao, Cao, Ren, Yu and Wang.

Two novel chlorinated and nitrogenated azaphilones, namely N-butyl-2-aza-2-deoxychaetoviridin A (1) and N-hexyl-2-aza-2-deoxychaetoviridin A (2), along with a previously identified analogue, chaetoviridin A (3), were successfully obtained from Chaetomium globosum 2020HZ23, a marine algal-sourced endophytic fungus. The planar structures as well as the absolute configurations of these new metabolites were determined utilizing a synergistic approach that involved both spectroscopic techniques (1D/2D NMR and HRESIMS) and Density Functional Theory (DFT) calculations. Each compound was subject to in vitro cytotoxicity evaluation toward the A549 cancer cell line. Both compounds 1 and 2 demonstrated significant cytotoxicity, as evidenced by their respective IC50 values of 13.6 and 17.5 μM. Furthermore, 1 and 2 demonstrated potent cell migration inhibition, which elevated with increasing dose concentration. In contrast, compound 3 exhibited less cytotoxic activity relative to 1 and 2, suggesting that the cytotoxic potency escalates with N-substitution at the C-2 position and the introduction of a side chain. This finding could offer implications for future studies aimed at designing and refining lead compounds within this class.Copyright © 2023 Gao, Cao, Ren, Yu and Wang.