引言：慢性髓性白血病（CML）是由BCR-ABL嵌合酪氨酸激酶引起的克隆性骨髓增殖性疾病。长春花碱（VCR）被广泛用于白血病治疗，但多药耐药性（MDR）是其制约因素。方法：我们通过自组装制备DNA纳米花来传递VCR和P-糖蛋白小干扰RNA（P-gp siRNA）。结果和讨论：所制备的纳米花形状呈花状，并具有较高的VCR（80%）负荷效率。此外，纳米花可以将VCR和P-gp siRNA传递到MDR CML细胞中，并在体外和体内诱导强效细胞毒性，从而克服CML的多药耐药性。总体而言，这种纳米花是抗耐药性CML治疗的有希望的工具。版权所有© 2023赵，钟，潘，张，田，张，易，赵和吴。

Introduction: Chronic myelogenous leukemia (CML) is a clonal myeloproliferative disorder caused by the BCR-ABL chimeric tyrosine kinase. Vincristine (VCR) is widely used in leukemia therapy but is hindered by multidrug resistance (MDR). Methods: We prepared DNA nanoflower via self-assembly for the delivery of VCR and P-glycoprotein small interfering RNA (P-gp siRNA). Results and Discussion: The as-prepared nanoflower had a floriform shape with high loading efficiency of VCR (80%). Furthermore, the nanoflower could deliver VCR and P-gp siRNA into MDR CML cells and induce potent cytotoxicity both in vitro and in vivo, thus overcoming MDR of CML. Overall, this nanoflower is a promising tool for resistant CML therapy.Copyright © 2023 Zhao, Zhong, Pan, Zhang, Tian, Zhang, Yi, Zhao and Wu.