本研究旨在优化壳聚糖稳定的Pickering乳液（PE），其中含有5-氟尿嘧啶（5-FU）作为潜在的鳞癌治疗药物。同时，采用紫外-可见分光光度法和反相高效液相色谱法（RP-HPLC）对5-氟尿嘧啶进行了全面分析，结果显示具有良好的线性度、灵敏度、精密度和稳健性。表征技术揭示了Pickering乳液（PE）的形态学特征、固体凝胶性质、成功的包封效果和良好的抗癌效果。傅里叶变换红外光谱（FTIR）用于验证包封的效力，差示扫描量热仪（DSC）用于确认后包封药物的稳定性。0.6%壳聚糖稳定的PE表现出良好的稳定性和药物负载效率。开发了抗EGFR-5-FU-CS-PE凝胶以实现持续药物释放，用于鳞癌的治疗。体外实验证明，抗EGFR-5-FU-CS-PE表现出强大的抗癌效果，IC50低于5-FU和5-FU-CS-PE。基于壳聚糖的抗EGFR-5-FU-PE Pickering乳液的流变特性、细胞相互作用和治疗潜力进行了研究。两种乳液和凝胶在成功包封后表现出持续的体外药物释放。抗EGFR-5-FU-CS-PE可诱导细胞凋亡，降低线粒体膜电位，抑制癌细胞迁移。还对Wistar小鼠进行了安全性和抑制肿瘤生长的测试。所有配方都表现出出色的六个月稳定性。抗EGFR-5-FU-CS-PE表现为可行的治疗选择，需要进一步研究。版权所有 © 2023. Elsevier B.V. 发布。

This research seeks to optimize a chitosan-stabilized Pickering emulsion (PE) containing 5-fluorouracil (5-FU) as a potential Squamous Cell Carcinoma therapy. The 5-Fluorouracil was also thoroughly analysed using UV spectrophotometry and RP-HPLC, demonstrating exceptional linearity, sensitivity, precision, and robustness. The techniques of characterization revealed Pickering emulsion (PE) morphology, solid-like gel properties, successful encapsulation, and promising anticancer effects. FTIR was used to validate the efficacy of encapsulation, and DSC was used to confirm the post-encapsulation drug stability. The 0.6 % chitosan-stabilized PE showed exceptional stability and drug loading efficiency. Anti-EGFR-5-FU-CS-PE gel was developed for sustained drug release in the treatment of Squamous Cell Carcinoma. Anti-EGFR-5-FU-CS-PE demonstrated potent anticancer effects in vitro, with a lower IC50 than 5-FU and 5-FU-CS-PE. Anti-EGFR-5-FU-PE Pickering emulsions based on chitosan were investigated for their rheological properties, cellular interactions, and therapeutic potential. Both emulsions and gel exhibited sustained in vitro drug release after successful encapsulation. Anti-EGFR-5-FU-CS-PE induced apoptosis, decreased mitochondrial membrane potential, and inhibited the migration of cancer cells. Wistar mice were tested for safety and tumour growth inhibition. All formulations exhibited exceptional six-month stability. Anti-EGFR-5-FU-CS-PE emerges as a viable therapeutic option, necessitating additional research.Copyright © 2023. Published by Elsevier B.V.