胶质母细胞瘤（GB）容易在手术后复发。因此，需要针对术后区域内的复发性GB细胞进行局部治疗。在本研究中，我们使用橄榄苷（OL）和芦丁以及它们与替莫唑胺（TMZ）的组合，开发了基于纳米纤维的局部治疗方法，针对GB细胞。通过电纺过程将聚乳酸（PLA）的核-壳纳米纤维网包裹于OL（PLAOL）、芦丁（PLArutin）和TMZ（PLATMZ）中。扫描电镜用于观察PLA纤维网的形态，全浸法确定其释放特性。采用实时细胞追踪分析细胞生长，采用双荧光染料曙红橙/碘化丙啶染色检测细胞活力，刮伤愈合实验评估细胞迁移能力，用球体形成实验评估肿瘤球体的侵袭性。所有聚合物纳米纤维网具有核-壳结构，平均直径在133±30.7-139±20.5nm之间。所有PLA纤维网均促进凋亡细胞死亡，抑制细胞迁移和球体生长（p < 0.0001）。PLAOL和PLATMZ通过控制释放，在120小时内抑制GB细胞的活力增加，而PLArutin导致快速的细胞抑制（p < 0.0001）。总的来说，我们的研究结果表明，核-壳纳米纤维网可以成为针对复发性GB细胞的橄榄苷和TMZ控制释放的新型有效治疗工具。版权©2023 Elsevier B.V.出版。

Glioblastoma (GB) has susceptibility to post-surgical recurrence. Therefore, local treatment methods are required against recurrent GB cells in the post-surgical area. In this study, we developed a nanofiber-based local therapy against GB cells using Oleuropein (OL), and rutin and their combinations with Temozolomide (TMZ). The polylactic acid (PLA) core-shell nanofiber webs were encapsulated with OL (PLAOL), rutin (PLArutin), and TMZ (PLATMZ) by an electrospinning process. A SEM visualized the morphology and the total immersion method determined the release characteristics of PLA webs. Real-time cell tracking analysis for cell growth, dual Acridine Orange/Propidium Iodide staining for cell viability, a scratch wound healing assay for migration capacity, and a sphere formation assay for tumor spheroid aggressiveness were used. All polymeric nanofiber webs had core-shell structures with an average diameter between 133 ± 30.7-139 ± 20.5 nm. All PLA webs promoted apoptotic cell death, suppressed cell migration, and spheres growth (p < 0.0001). PLAOL and PLATMZ suppressed GB cell viability with a controlled release that increased over 120 h, while PLArutin caused rapid cell inhibition (p < 0.0001). Collectively, our findings suggest that core-shell nano-webs could be a novel and effective therapeutic tool for the controlled release of OL and TMZ against recurrent GB cells.Copyright © 2023. Published by Elsevier B.V.