近年来，多功能纳米颗粒在癌症治疗中受到越来越多的关注。在这项研究中，我们采用了一种新型的聚多巴胺（PDA）框架基金葡萄糖酸胺改性金纳米颗粒作为抗代谢药物5-氟尿嘧啶（5-FU）的载体。叶酸（FA）被点缀在纳米颗粒表面，通过与丰富表达于乳腺癌细胞中的叶酸受体的精确结合，赋予纳米系统显著的肿瘤靶向能力。PDA作为光热治疗（PTT）剂和控制药物释放的门卫，因其高度pH敏感性，可以延长纳米材料的停留时间，同时增强水溶性和生物相容性。金纳米颗粒（Au NPs）作为药物传递平台和大量光热效应的源头，最终实现了协同化疗光热疗法。PDA@Au@FA纳米复合物装载了5-FU，具有良好的生物相容性、强大的近红外吸收和光热转化能力，并能在pH/近红外双响应环境中控制药物释放。在具有NIR照射的48小时内，PDA@Au@5-FU-FA组的细胞存活率仅为20.1±2.6%。此外，凋亡染色实验显示，MCF-7细胞对PDA@Au@5-FU-FA的摄取更多。因此，我们在此提出的PDA@Au@5-FU-FA纳米复合物可能成为乳腺癌有效治疗的潜在候选者。版权所有 © 2023。Elsevier B.V.出版。

Multifunctional nanoparticles for treatment in cancer are getting more and more attention recently. In this study, we employed a novel polydopamine (PDA) framework-based gold nanoparticles as a carrier of an antimetabolite drug, 5-Fluorouracil (5-FU). Folic acid (FA) was embellished onto the surface of nanoparticle imparting the nanosystem with remarkable tumor-targeting abilities through its precise binding with FA receptor that is notably overexpressed in breast cancer cells. PDA served as a photothermal treatment (PTT) agent and a gatekeeper to regulate drug release since it is highly pH-sensitive and might lengthen the residency period while simultaneously enhancing water solubility and biological compatibility of nanomaterials. Gold nanoparticles (Au NPs) end up serving as both a drug delivery platform and a source of substantial photothermal effects, culminating in synergistically coupled chemo-photothermal therapy. The PDA@Au@FA nanocomplex, loaded with 5-FU, is biocompatible, features strong NIR absorption and photothermal conversion, and can control drug release in pH/NIR dual response environment. The cell viability in PDA@Au@5-FU-FA group with NIR irradiation in 48 h was only 20.1±2.6%. In addition, apoptosis staining experiments revealed greater cellular uptake of PDA@Au@5-FU-FA by MCF-7 cells. Therefore, PDA@Au@5-FU-FA nanocomplex that we postulated herein may be a potential contender for effective curative treatment for breast cancer.Copyright © 2023. Published by Elsevier B.V.