对从进行颌面部放射治疗的肿瘤患者体内提取的白念珠菌属种类，评估并比较伏立康唑与制霉菌素的抗真菌效果。
Evaluation and comparison of antifungal effect of voriconazole with nystatin on candida species derived from neoplastic patients undergoing maxillofacial radiotherapy.
发表日期：2023
作者：
Faezeh Khozeimeh, Zahra Golestan Nezhad, Parvin Dehghan, Adel Tabesh, Mahnaz Kheirkhah, Mina Jamshidi, Ahmad Amiri Chermahini, Leila Sadeghalbanaei, Zahra Golestannejad
来源：
Parasites & Vectors
摘要：
放射治疗是头颈部恶性肿瘤的常见治疗方法，但也会引起口腔念珠菌病和口腔白色念珠菌转变为非白色念珠菌等并发症。伏立康唑具有可接受的抗真菌效果。本研究旨在确定并比较酮酸康与伏立康唑对这些菌株的抗真菌效果。在Seyed Al-Shohada医院进行了这项体外研究，样本通过聚合酶链反应-限制性片段长度多态性鉴定方法，从头颈部放疗患者中选取，分别于放疗前和放疗后的2周取得。采用微量稀释法确定了酮酸康和伏立康唑的抗真菌效果，并测量了最低抑菌浓度（MIC）和最低杀菌浓度，结果通过曼-惠特尼分析进行分析。结果显示，放疗前后的所有菌株对酮酸康表现出100%的敏感性。在放疗前，分离出的白色念珠菌中，57.1%位于敏感范围（MIC≤1），42.9%位于剂量依赖范围（MIC=2）对伏立康唑敏感。放疗后，58.3%的白色念珠菌处于敏感范围，41.7%的菌株处于对伏立康唑的剂量依赖范围。本研究结果显示，在放疗前，所有菌株对酮酸康敏感，但部分白色念珠菌和非白色念珠菌对伏立康唑存在抗药性。在放疗第2周，与放疗前类似，所有患者分离出的菌株对酮酸康敏感，但部分白色念珠菌和非白色念珠菌对伏立康唑存在抗药性。
版权所有：© 2023 Dental Research Journal。
Radiotherapy is a common treatment for head-and-neck malignancies and causes complications such as oral candidiasis and the change of oral Candida species from albicans to nonalbicans. Voriconazole has acceptable antifungal effect. The aim of this study was to determine and compare the antifungal effect of nystatin with voriconazole on these species.The samples used in this in vitro study were identified by polymerase chain reaction-restriction fragment length polymorphism from patients before and 2 weeks after head-and-neck radiotherapy in Seyed Al-Shohada Hospital. The antifungal effect of nystatin and voriconazole was determined by microdilution method and measurement of the minimum inhibitory concentration (MIC) and the minimum fungicidal concentration, and the results were analyzed by Mann-Whitney analysis.The results showed that all species before and after radiotherapy showed 100% sensitivity to nystatin. Prior to radiotherapy, 57.1% of albicans species isolated were in the sensitive range (MIC ≤1) and 42.9% were in the dose-dependent range (MIC = 2) to voriconazole. After radiotherapy, 58.3% of albicans species were in the sensitive range and 41.7% of these species were in the dose-dependent range to voriconazole.The results of the present study showed that before radiotherapy, all species were sensitive to nystatin, while a percentage of albicans and nonalbicans were resistant to voriconazole. In the 2nd week of radiotherapy similar to prior to radiotherapy, all species isolated from patients were sensitive to nystatin, while a percentage of albicans and nonalbicans were resistant to voriconazole.Copyright: © 2023 Dental Research Journal.