缺血再灌注损伤（IRI）是急性和慢性肾衰竭、心脏衰竭和缺血性中风的主要原因。本研究旨在研究Iberin的治疗潜力，Iberin以其抗炎、抗氧化和抗凋亡特性而闻名，研究以大鼠肾脏IRI模型为基础。将24只成年雄性大鼠随机分为四组：I组（假手术组）进行腹腔镜手术而无产生IRI；II组（对照组）进行腹腔镜手术，停止肾动脉灌注30分钟以诱导缺血，然后进行2小时再灌注；III组（Iberin治疗组）注射Iberin（15 mg/kg），进行30分钟缺血，然后进行2小时再灌注；IV组（车辆处理组）在缺血和再灌注诱导前1小时给予乙醇溶剂（适量）。Iberin用乙醇稀释。使用酶联免疫吸附测定法测量与炎症、氧化应激和凋亡相关的生物标志物。Iberin治疗显著降低了炎性细胞因子白介素1β（IL-1β）和IL-6、Bcl-2相关X蛋白（BAX）、肿瘤坏死因子α（TNF-α)、核因子kappa p56、高迁移率组蛋白B1以及嗜中性粒细胞胶原酶相关脂蛋白的水平。此外，与对照组和车辆组相比，Iberin增加了热休克蛋白和Bcl-2的水平。Iberin治疗延长了肾脏组织的缺血耐受性，潜在地预防或延缓了不可逆损伤。这些发现凸显了Iberin作为调节炎症标志物的潜在候选药物，用于缓解由缺血再灌注引起的肾脏损伤的潜力。©2023 JOURNAL of MEDICINE and LIFE.

Ischemia-reperfusion injury (IRI) is a major contributor to acute and chronic kidney failure, heart failure, and ischemic stroke. This study aimed to investigate the therapeutic potential of Iberin, known for its anti-inflammatory, antioxidant, and antiapoptotic properties, in a rat model of renal IRI. Twenty-four adult male rats were randomly divided into four groups: Group I (Sham group) underwent laparotomy without IRI induction; Group II (Control group) underwent laparotomy followed by renal artery clamping for 30 minutes to induce ischemia, followed by 2 hours of reperfusion; Group III (Iberin treatment group) received a pre-injection of Iberin (15 mg/kg) and underwent 30 minutes of ischemia followed by 2 hours of reperfusion; and Group IV (Vehicle-treated group) received the vehicle (ethanol) 1 hour prior to ischemia and reperfusion induction. Iberin was diluted with ethanol. Biomarkers associated with inflammation, oxidative stress, and apoptosis were measured using enzyme-linked immunosorbent assay. Iberin treatment significantly reduced levels of inflammatory cytokines interleukin-1β (IL-1β) and IL-6, Bcl-2-associated X protein (BAX), tumor necrosis factor α (TNF-α), nuclear factor kappa p56, high mobility group B1, and neutrophil gelatinase-associated lipocalin. Moreover, Iberin increased levels of heat shock protein and Bcl2 compared to the control and vehicle groups. Iberin treatment prolonged the ischemic tolerance of renal tissue, potentially preventing or delaying irreversible injuries. These findings highlight the potential of Iberin as a promising candidate for mitigating renal injury caused by ischemia-reperfusion, due to its ability to modulate inflammatory markers.©2023 JOURNAL of MEDICINE and LIFE.