免疫疗法被认为是一种有前途的癌症治疗策略。然而，只有少数头颈部鳞状细胞癌患者对免疫疗法产生积极反应。本研究旨在发现头颈部鳞状细胞癌可能的抗原，为该类型癌症制备mRNA疫苗，并研究头颈部鳞状细胞癌与牙周病之间的关联，以及确定头颈部鳞状细胞癌受影响的免疫细胞亚型。为了确定与之对应的基因表达谱和临床数据，我们在TCGA数据库上进行了检查。使用TIMER检测到了抗原呈递细胞。针对头颈部鳞状细胞癌的六个免疫相关基因（CXCL5、ADM、FGF9、AIMP1、STC1和CDKN2A）的靶向治疗已经在由牙周病引发的免疫疗法中显示出有希望的结果。这些基因与改善预后和增加免疫细胞浸润有关。此外，CXCL5、ADM、FGF9、AIMP1、STC1和CDKN2A在制备mRNA疫苗时也显示出了潜力作为抗原。我们建立了一个包含ADM表达和肿瘤分期的临床实践中的Nomogram模型。总之，ADM显示出潜力作为预测头颈部鳞状细胞癌细胞预后、分子特征和免疫特性的候选生物标志物。我们通过实时PCR分析获得的结果显示，与NOK-SI细胞系相比，SCC-25细胞系中ADM显著上调，这表明ADM可能与HNSC的发病机制有关，突显了ADM在HNSC治疗中的潜力靶点。然而，需要进一步研究ADM在HNSC中的功能作用。我们的研究结果为深入探索HNSC分子机制并帮助开发新的治疗策略提供了基础。版权所有 © 2023 Fu和Zheng。

Among cancer treatments, immunotherapy is considered a promising strategy. Nonetheless, only a small number of individuals with head and neck squamous cell carcinoma exhibit positive responses to immunotherapy. This study aims to discover possible antigens for head and neck squamous cell carcinoma, create an mRNA vaccine for this type of cancer, investigate the connection between head and neck squamous cell carcinoma and periodontal disease, and determine the immune subtype of cells affected by head and neck squamous cell carcinoma. To ascertain gene expression profiles and clinical data corresponding to them, an examination was carried out on the TCGA database. Antigen-presenting cells were detected using TIMER. Targeting six immune-related genes (CXCL5, ADM, FGF9, AIMP1, STC1, and CDKN2A) in individuals diagnosed with head and neck squamous cell carcinoma has shown promising results in immunotherapy triggered by periodontal disease. These genes have been linked to improved prognosis and increased immune cell infiltration. Additionally, CXCL5, ADM, FGF9, AIMP1, STC1, and CDKN2A exhibited potential as antigens in the creation of an mRNA vaccine. A nomogram model containing ADM expression and tumor stage was constructed for clinical practice. To summarize, ADM shows potential as a candidate biomarker for predicting the prognosis, molecular features, and immune characteristics of head and neck squamous cell carcinoma cells. Our results, obtained through real-time PCR analysis, showed a significant upregulation of ADM in the SCC-25 cell line compared to the NOK-SI cell line. This suggests that ADM might be implicated in the pathogenesis of HNSC, highlighting the potential of ADM as a target in HNSC treatment. However, further research is needed to elucidate the functional role of ADM in HNSC. Our findings provide a basis for the further exploration of the molecular mechanisms underlying HNSC and could help develop novel therapeutic strategies.Copyright © 2023 Fu and Zheng.