引言：C-藻蓝蛋白（C-PC）是从螺旋藻中获得的光合蛋白，被认为是一种极具商业价值的生物化学品。大量的体外和体内研究已经证实了C-PC减轻炎症反应、缓解氧化应激和促进伤口愈合的能力。然而，尽管已经存在有关C-PC对紫外线B（UVB）诱导的细胞凋亡的保护机制的认识，还需要更多的体内实验来探索其抗光老化机制。方法：本研究使用BALB/c-nu小鼠建立了UVB诱导的皮肤光老化模型，并通过各种分子生物学工具研究了局部应用C-PC的潜在保护作用。此外，还开发了一种新型输送系统，C-PC纳米分散液，以促进C-PC的经皮传递。结果：C-PC在UVB诱导的皮肤中显示出显著的抗光老化活性。将C-PC应用于小鼠的背部皮肤，在UVB照射下改善了宏观特征，如下垂和粗糙皱纹的减少。组织学分析表明，C-PC治疗显著减少了表皮增厚的症状，防止了真皮胶原纤维松弛，增加了小鼠皮肤中羟脯氨酸（Hyp）含量和抗氧化酶活性（如超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶），降低了丙二醛水平和炎症因子（白细胞介素-1α、白细胞介素-1β、白细胞介素-6和肿瘤坏死因子-α）的表达，降低了基质金属蛋白酶（MMP-3和MMP-9）的表达，并抑制了丝裂原活化蛋白激酶家族中的c-Jun N末端激酶、细胞外信号调节激酶和p38蛋白的磷酸化。讨论：通过分析研究结果，我们提出了一种新的药物传递系统C-PC纳米分散液，并研究了C-PC的抗光老化作用及其机制。版权所有 © 2023 Zhou, Bai, Huang, Li, Chen, Cheng, Wu and Diao.

Introduction: C-phycocyanin (C-PC), a photosynthetic protein obtained from Spirulina, is regarded a highly promising commercially available biochemical. Numerous in vitro and in vivo studies have provided evidence of C-PC's ability to mitigate the inflammatory response, alleviate oxidative stress, and facilitate wound healing. However, despite the existing knowledge regarding C-PC's protective mechanism against cellular apoptosis induced by ultraviolet B (UVB) radiation, further in vivo experiments are needed to explore its anti-photoaging mechanism. Methods: In this study, a UVB-induced skin photoaging model was established using BALB/c-nu mice, and the potential protective effects of topically administered c-PC were investigated by various molecular biology tools. In addition, a novel delivery system, C-PC nanodispersion, was developed to facilitate the transdermal delivery of C-PC. Results: C- PC demonstrated significant anti-photoaging activities in the UVB-induced skin. The application of C-PC to the dorsal skin of the mice resulted in improved macroscopic characteristics, such as reduced sagging and coarse wrinkling, under UVB irradiation Histological analyses showed that C-PC treatment significantly decreased the symptoms of epidermal thickening, prevented dermal collagen fiber loosening, increased the hydroxyproline (Hyp) content and activities of antioxidant enzymes (such as superoxide dismutase, catalase, and glutathione peroxidase) in mouse skin, decreased malondialdehyde levels and expressions of inflammatory factors (interleukin-1α [IL-1α], IL-1β, IL-6, and tumor necrosis factor-α), reduced matrix metalloproteinase [MMP-3 and MMP-9] expressions, and inhibited the phosphorylation of c-Jun N-terminal kinase, extracellular signal-regulated kinase, and p38 proteins in the mitogen-activated protein kinase family. Discussion: By analyzing the results of the study, a new drug delivery system, C-PC nano-dispersion, was proposed, and the anti-photoaging effect of C-PC and its mechanism were investigated.Copyright © 2023 Zhou, Bai, Huang, Li, Chen, Cheng, Wu and Diao.